2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol

Triton-X has been used:

• In the permeabilization of cells for immunofluorescence staining
• As a component of lysis buffer in western blot analysis
• As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Triton X-100 has been used:

• In immunohistochemistry for staining the Flat-mount retinas
• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
• To permeabilise cells during Immunofluorescent microscopic studies
• As a positive control in LDH assay to determine the cell membrane integrity
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
• For the preparation of outer membrane protein exctract
• As a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cells
• In the treatment of tissue sections for Immunofluorescence labeling

0·3% Triton X-100 in TBS has been used for permeabilization of retinas before immunostaining. 0.5% Triton X-100 has been used for astrocyte cell lysis.

Octophenolpoly(ethyleneglycolether)_n
Especially used for purification in membrane research
Triton X-100 is a water soluble, non-ionic surfactant. It exhibits a strong hydrogen bonding with water molecule in temperature dependent manner. It has a wide application area such as in isolation and solubilization of protein, DNA extraction and emulsification.

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Moderately toxic by ingestion.When heated to decomposition it emits acrid smoke andirritating fumes.

Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits acridsmoke and irritating fumes.

The initial threshold screening level (ITSL) for polyethylene glycol mono(octylphenyl)ether is 1.9 μg/m3 based on an annual averaging time.