基本属性 生物活性体外研究 用途与合成方法 MSDS 荧光素二乙酸盐 价格(试剂级) 供应商 供应信息 相关产品
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荧光素二乙酸盐

荧光素二乙酸盐

英文名称:Fluorescein diacetate
CAS号:596-09-8
分子式:C24H16O7
分子量:416.38
EINECS号:209-877-6
Mol文件:596-09-8.mol
荧光素二乙酸盐 结构式

荧光素二乙酸盐 性质

熔点 200-203 °C(lit.)
沸点 494.73°C (rough estimate)
密度 1.2738 (rough estimate)
折射率 1.6810 (estimate)
储存条件 −20°C
溶解度 丙酮:可溶25mg/mL
形态 结晶粉末
酸度系数(pKa) 6.4(at 25℃)
颜色 浅黄色
酸碱指示剂变色ph值范围 Weak green uorescence (6.0) to strong green uorescence (7.2)
水溶解性 Soluble in chloroform, DMSO, ethanol, and water.
最大波长(λmax) 490nm
BRN 62575
主要应用 LED devices, petroleum markers, identifying cancerous cells, detecting bacteria, virus, mitochondrial function in neurons, target molecules, living cells, viable Giardia cysts in water samples, apoptosis, evaluating live/dead state of algae, measurement of totalmicrobial activity in soil, treating ischemia
生物领域应用 Assessing membrane integrity/permeability;43–50 detecting/counting microorganisms; measuring microbial activity; as a substrate for measuring acylases activity, amidases activity,aminopeptidases activity, arylsulfatases activity,chymotrypsin activity, esterases activity;galactosidases activity, glucosidases activity, lipases activity, phosphatases activity, proteases activity; cell viability assay; cytotoxicity assay; implantable medical devices; as temperature sensor; treating ischemic disease
CAS 数据库 596-09-8(CAS DataBase Reference)
EPA化学物质信息 Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)- (596-09-8)

荧光素二乙酸盐 用途与合成方法

Fluorescein diacetate 是一种细胞渗透性的酯酶底物。Fluorescein diacetate 可作为人谷胱甘肽硫转移酶 Pi (hGSTP1-1) 的荧光底物。

Fluorescein diacetate (FDA) is an acetylated derivative of the green fluorescent dye fluorescein. Fluorescein diacetate (FDA), a fluorescent probe used for vital staining, is a fluorescently activated by esterolytic activity of human Pi-class glutathione S-transferase (hGSTP1) selectively among various cytosolic GSTs. Fluorescence activation of Fluorescein diacetate susceptible to GST inhibitors is observed in MCF7 cells exogenously overexpressing hGSTP1, but not in cells overexpressing hGSTA1 or hGSTM1. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1. To investigate whether the fluorescence activation is due to hGSTP1 activity, Fluorescein diacetate is incubated with recombinant hGSTP1-1 and GSH in vitro. Remarkable fluorescence activation is observed in the presence of both hGSTP1-1 and GSH, whereas only slight activation is observed in the absence of either of them or when the enzyme is heat inactivated. This suggests that the fluorescence activation of Fluorescein diacetate depends on hGSTP1-1 activity. From the linear relationship between the rate of increase in fluorescence and the hGSTP1-1 concentration, the specific activity of the enzyme for 1 μM Fluorescein diacetate is determined to be 79±15 nmol/min/mg protein. Fluorescein diacetate is applicable as a fluorogenic substrate for evaluating inhibitors of GSTP1-1 in vitro. For Fluorescein diacetate as a substrate, both Ethacrynic acid (EA) and NBDHEX suppress the hGSTP1-1-dependent fluorescent increase in a concentration-dependent manner, with IC 50 s of 3.3±0.5 μM and 0.61±0.04 μM, respectively.

用途 
也叫做FDA,可透过细胞膜并作为荧光素在活细胞内积累。由于荧光素较BCECF或钙黄绿素的亲水性低,荧光素从细胞中渗漏的量也高。FDA也用于流式细胞仪。荧光素的激发和发射波长分别为488nm和530nm。
用途 
酯酶底物。FDA可透过细胞膜并作为荧光素积蓄在活细胞内。由于荧光素较BCECF或Calcein的亲水性低,因此荧光素从细胞中渗漏的量也高。FDA也可用于流式细胞仪。荧光素的激发和发射波长分别为488 nm和530 nm。

安全信息

危险品标志Xi
危险类别码36/37/38
安全说明24/25-36/37/39-27-26
WGK Germany3
TSCAYes
海关编码32129000

MSDS信息

语言:English
提供商:ACROS
语言:English
提供商:SigmaAldrich
语言:Chinese
提供商:ALFA
语言:English
提供商:ALFA

荧光素二乙酸盐 价格(试剂级)

更新日期 产品编号 产品名称 CAS号 包装 价格
2024-01-25 HY-D0719 荧光素二乙酸盐 596-09-8 500mg 400
2024-01-25 HY-D0719 荧光素二乙酸盐 596-09-8 5G 500

荧光素二乙酸盐供应商 更多

靖江市东尼化工产品有限公司
黄金产品
联系电话:0510-85911828 18068357565
产品介绍:
中文名称:荧光素二乙酸酯
英文名称:FLUORESCEIN DIACETATE
CAS:596-09-8
纯度:99%
包装信息:1KG;50KG;
湖南汇百益新材料有限公司
现货
联系电话:0745-2866551 19374576088
产品介绍:
中文名称:二乙酸荧光素
英文名称:Fluorescein diacetate
CAS:596-09-8
纯度:99%
包装信息:按需包装
备注:源头工厂,现货、可定制、大货、可分装,价格更具优势
库存量:1000g
现货日期:2024/3/15 10:23:49
北京百灵威科技有限公司
联系电话:010-82848833 400-666-7788
产品介绍:
中文名称:荧光素二乙酸酯
英文名称:Fluorescein diacetate, 97%
CAS:596-09-8
纯度:97%
包装信息:1G;25G;5G
备注:化学试剂、精细化学品、医药中间体、材料中间体
上海迈瑞尔生化科技有限公司
联系电话:4006608290; 18621169109
产品介绍:
中文名称:荧光素二乙酸酯
英文名称:Fluorescein Diacetate
CAS:596-09-8
纯度:>98.0%(LC)
备注:F0240
阿法埃莎(中国)化学有限公司
联系电话:400-6106006
产品介绍:
中文名称:荧光素二乙酸盐, 97%
英文名称:Fluorescein diacetate, 97%
CAS:596-09-8
包装信息:5g
备注:B24466

荧光素二乙酸盐生产厂家及价格列表

FDA
上海康朗生物科技有限公司 2024-03-19
二乙酸萤光素
上海康朗生物科技有限公司 2024-03-19

"荧光素二乙酸盐"相关产品信息