Intracellular, cytosolic, low-molecular
weight, metal-bind; consists of two similar lowmolecular-weight moieties; high cysteine content.
Inducible protein that is
involved in the detoxification of heavy metals.
Purify it by precipitation to give Zn-and Cd-containing protein fractions and running it on a Sephadex G-75 column, then isoelectric focusing to give two protein peaks [Nordberg et al. Biochem J 126 491 1972, Comeau et al. Prep Biochem 22 151 1992].