Cytidine 5'-monophosphate disodium salt (5'-CMPNa2) is an organic sodium salt that can be used as a pharmaceutical active ingredient. 5'-CMPNa2 can be used with UMP-Na2, vitamin B6, lipoic acid, and magnesium lactate dihydrate to prepare a capsule-type compound drug for the treatment of polyneuropathy[1].
The production method of Cytidine 5'-monophosphate disodium salt comprises the following steps: a. adding cytidine, triethyl phosphate and pyridine into a reaction container respectively, and adjusting the reaction temperature to -15-5°C after mixing evenly, and slowly adding a certain amount of phosphorus oxychloride under the temperature condition, and continuing to stir and react for 10-30h after adding; b. taking samples for HPLC analysis, and when the conversion rate is ≥99%, adding 10-15% ice salt water for hydrolysis, and the hydrolysis temperature is controlled to be 0-5°C, and the hydrolysis time is 10-20h; c. after the hydrolysis is completed, standing and stratifying, removing the organic phase, and obtaining a water layer for standby use; d. adjusting the pH of the water layer to 7.0-8.0 with 30% sodium hydroxide, and then dripping 95% alcohol twice the volume of the solution, precipitating crystals, and filtering to obtain a crude product. e. dissolving the crude product, adding activated carbon for decolorization, and continuing to drip 95% alcohol twice the volume of the solution to precipitate crystals, filtering, rinsing, and drying to obtain the finished product Cytidine 5'-monophosphate disodium salt.
