Sephadex G-25 is a gel filtration medium used in affinity chromatography, protein chromatography and gel filtration chromatography.
Fractionation Range (MW)
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000
It has been used in the desalting of protease and in the column for the separation of radioiodinated oTP-1 from free iodine during the characterization of radioiodinated oTP-1 and receptor assay validation.
Sephadex G-25 is suitable for buffer exchange and desalting oligonucleotides for use in sequencing, PCR, and labeling applications. It was used to isolate two different peptides of Pronase E extracted from Alaska Pollack skin.
G25300-100G′s updated product number is GE17-0034-01
G25300-500G′s updated product number is GE17-0034-02
Sephadex(R) G-25 is a well-established gel filtration resin, widely used for desalting and buffer exchange in industrial applications. Its hydrophilic matrix minimizes nonspecific adsorption, resulting in high recoveries during these processes for proteins and nucleic acids. Sephadex(R) G-25 is usually used in affinity chromatography, protein chromatography and gel filtration chromatography.
