Nuclear Fast Red is used as a biological stain and is suitable for the determination of calcium. Nuclear Fast Red targets nucleic acids and works more quickly than Hematoxylin. Dyes and metabolites.
ChEBI: An organic sodium salt that is the monosodium salt of 4-amino-1,3-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid. It forms bright red lakes with calcium and is a useful red nuclear staining lake with aluminum.
Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei.
A solution of 5g of the dye in 250mL of warm 50% EtOH is cooled to 15o for 36hours, then filtered on a Büchner funnel, washed with EtOH until the washings are colourless, then with 100mL of Et2O and dry it over P2O5 in vacuo. It is a biological stain which is also used for the estimation of Ca. [Kingsley & Robnett Anal Chem 33 552 1961.] Octadecyl isonicotinate See hydrogen ionophore IV, ETH 1778 in “Heterocyclic Compounds”, Chapter 4.