N-[トリス(ヒドロキシメチル)メチル]グリシン

化学名:N-[トリス(ヒドロキシメチル)メチル]グリシン

CAS番号.5704-04-1

英語名:Tricine

CBNumberCB5853856

MFC6H13NO5

MW179.17

别名

N-[トリス(ヒドロキシメチル)メチル]グリシン

N-[1,1-ビス(ヒドロキシメチル)-2-ヒドロキシエチル]グリシン

トリシン

N-[トリス(ヒドロキシメチル)メチル]グリシン物理性質

融点	186-188 °C (dec.)
沸点	311.69°C (rough estimate)
比重(密度)	1.05 g/mL at 20 °C
かさ密度	500kg/m3
蒸気圧	0.002-0.004Pa at 20-25℃
屈折率	1.4240 (estimate)
貯蔵温度	Keep in dark place,Inert atmosphere,Room temperature
溶解性	H2O: 4M at20℃、透明、無色
外見	結晶性
色	無色透明 (40% (w/w) in H₂O) 溶液
PH	4.0-6.0 (1M in H2O)
酸解離定数(Pka)	8.1(at 25℃)
酸塩基指示薬変色域(pH)	7.4 - 8.8
臭い (Odor)	無臭
由来生物	synthetic
水溶解度	溶ける
Merck	14,9651
BRN	1937804
安定性：	安定。
InChI	1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChIKey	SEQKRHFRPICQDD-UHFFFAOYSA-N
SMILES	OCC(CO)(CO)NCC(O)=O
LogP	-2.87 at 25℃
CAS データベース	5704-04-1(CAS DataBase Reference)
EPAの化学物質情報	Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)
Absorption	≤0.05 at 280 in H2O at 1M ≤0.1 at 260 in H2O at 1M

安全性情報

危険有害性情報のコード(GHS)

外観
白色結晶性粉末
溶解性
水に溶け(8.95g/50ml水)、0℃において0.8mol/lで飽和する。有機溶媒には溶けない。
化学的特性
White/clear crystalline powder
使用
Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets. It is a zwitterionic amino acid commonly useful in the pH range of 7.4-8.8 and the pKa is 8.05 at 25°C. This buffer is also used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).
一般的な説明
Tricine and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood.
生物活性
Electrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.
純化方法
Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]

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