ChemicalBook > CAS DataBase List > Z-VAD-FMK

Z-VAD-FMK

CAS No.187389-52-2
Chemical Name:Z-VAD-FMK
CBNumber:CB0203438
Molecular Formula:C22H30FN3O7
Formula Weight:467.49
MOL File:Mol file

Z-VAD-FMK Property

Melting point >190°C (dec.)
Boiling point 732.4±60.0 °C(Predicted)
Density 1.214±0.06 g/cm3 (20 ºC 760 Torr)
Flash point 396.7℃
storage temp. -20°C
solubility DMSO: 20 mg/mL
form powder
pka 11.05±0.46(Predicted)
color white
biological source synthetic
Stability Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 1 week.
InChIKey MIFGOLAMNLSLGH-QOKNQOGYSA-N
SMILES C(N)(=O)[C@H](C)N(C(=O)[C@H](C(C)C)NC(OCC1=CC=CC=C1)=O)[C@@H](CC(OC)=O)C(=O)CF
CAS DataBase Reference 187389-52-2
UNSPSC Code 12352209
NACRES NA.77

Safety

Safety Statements :22-24/25
WGK Germany :3
HS Code :29145090

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordWarning
Hazard statements H315-H319
Precautionary statements P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313

Z-VAD-FMK Price More Price(4)

Brand: Sigma-Aldrich(India)
Product number: V116
Product name : Z-VAD-FMK
Purity: solid
Packaging: 2MG
Price: ₹87628.38
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: 627610
Product name : Caspase Inhibitor I - CAS 187389-52-2 - Calbiochem
Purity: Z-VAD-FMK, CAS 187389-52-2, is a cell-permeable, irreversible, pan-caspase inhibitor.
Packaging: 1MG
Price: ₹32810
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: SAB2501669
Product name : Anti-CASP3 antibody produced in goat
Purity: affinity isolated antibody, buffered aqueous solution
Packaging: 100μG
Price: ₹53135.7
Updated: 2022/06/14
Buy: Buy

Brand: Sigma-Aldrich(India)
Product number: 627610
Product name : Caspase Inhibitor I - CAS 187389-52-2 - Calbiochem
Purity: Z-VAD-FMK, CAS 187389-52-2, is a cell-permeable, irreversible, pan-caspase inhibitor.
Packaging: 5MG
Price: ₹57700
Updated: 2022/06/14
Buy: Buy

Z-VAD-FMK Chemical Properties,Usage,Production

Description Z-VAD-FMK (187389-52-2), cell-permeable, methyl ester form. Potent, irreversible pan-caspase inhibitor. Inhibits caspase activity and apoptosis induction in a variety of cell types (IC50 = 1.5 μM). Active in vivo.
Uses Z-VAD(OMe)-FMK is a cell-permeable, competitive, and irreversible inhibitor of all caspases. Through this action, it inhibits cleavage of poly(ADP-ribose) polymerase, preventing apoptosis when used at 10-50 μM. It also blocks caspase-mediated apoptosis in vivo. Z-VAD(OMe)-FMK effectively prevents caspase action in inflammasomes.[Cayman Chemical]
Uses Z-VAD-FMK is a caspase inhibitor that inhibits apoptosis and caspase processing in Jurkat T cells treated with low concentrations of z-FA-CMK.
Definition ChEBI: Z-Val-Ala-Asp(OMe)-CH2F is a tripeptide consisting of Z-Val-Ala-Asp(OMe) in which the C-terminal OH group has been replaced by a fluoromethyl group. An irreversible pan-caspase inhibitor. It has a role as an apoptosis inhibitor and a protease inhibitor. It is a carbamate ester, a tripeptide and an organofluorine compound.
General Description A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.
Biological Activity Cell-permeable, irreversible pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC 50 = 0.0015-5.8 mM). Active in vivo .
Biochem/physiol Actions Cell permeable: yes
Enzyme inhibitor This tripeptide halomethyl ketone (FWfree-acid = 467.49 g/mol; Soluble to 9.35 mg/ml in DMSO; CAS RegistryNumber = 187389-52-2), also known as Z-VAD-FMK and caspase inhibitor VI, is a broad-spectrum caspase inhibitor that blocks caspase-mediated apoptosis. Z-VAD-FMK inhibits caspase processing (IC50 = 0.0015–5.8 mM, depending on enzyme and cell type). The aspartate methyl ester derivative (FW = 467.49 g/mol), also known as caspase inhibitor I, is far more cell-permeable. Target (s) : caspases; caspase-1; caspase-2; caspase-3; caspase-4 ; caspase 5; caspase-6; caspase-7; caspase-8 ; caspase-9; caspase-10.
storage -20°C
References 1) Slee et al. (1996), Benzyloxycarbonyl-Val-Ala-ASP (OMe) fluoromethylketone (Z-VAD-FMK) inhibits apoptosis by blocking the processing of CPP32; Biochem. J., 315 21 2) Kunstle et al. (1997), ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-alpha; Immunol. Lett., 55 5 3) Garcia-Calvo et al. (1998), Inhibition of human caspases by peptide based and macromolecular inhibitors; J. Biol. Chem., 273 32608