Description
Creatine phosphate belongs to the human body and is the most important type of energy supplier. It can provide energy support for various tissues and organs. In human brain, kidney, cardiac muscle and skeletal muscle, 80% of the energy source is creatine phosphate. In humans, the synthesis of creatine phosphate begins in the kidney organs. A guanidinic acid is formed from arginine and glycine, and then creatine is formed through methylation in the liver. Finally, it is phosphorylated in various cellular tissues. And creatine phosphate is formed. Creatine phosphate disodium salt is a commercial creatine phosphate drug, which has become more and more widely used in acute myocardial infarction in recent years, and has achieved good results.
Uses
Creatine phosphate disodium salt is a white needle-like crystalline powder that is easily soluble in water. In medicine, it can protect the heart muscle by injecting creatine phosphate disodium salt, and can also be used to treat myocardial abnormalities in the ischemic state. creatine phosphate plays an important role in energy metabolism during muscle contraction[3]. The use of creatine phosphate is generally by intravenous injection. Phosphocreatine disodium salt exists as creatine phosphate after entering the body.
Chemical Properties
white powder
Uses
Creatine Phosphate Disodium Salt is an impurity of Creatine(C781483). Creatine can be used in biological study of high fat diets with different ketogenic ratios on hippocampal accumulation of creatine.
Biological Activity
Creatine Phosphate Sodium Salt is a high-energy phosphate compound that serves as a substrate for the determination of creatine kinase, which is used to regenerate ATP during skeletal muscle contraction. It is usually an integral part of the ATP-generating system. As long as enough ATP is present, it can store energy from the mitochondria. When ATP levels are low, the energy from creatine phosphate is transferred to the ADP molecule and then rapidly converted into ATP.
Purification Methods
The salt first appears as oily droplets which slowly settle and crystallise. After 12hours the supernatant is clear. Stirring and scratching the flask containing the filtrate brings out additional crystals (0.3-1g) if the salt is kept at 20o for 12hours. Filter it off at room temperature, wash with 3 x 5mL of ice-cold 90% EtOH, then 5mL of absolute EtOH and dry it in a vacuum desiccator (Drierite or CaCl2) for 16-30hours. The hexahydrate (plates) is converted to the tetrahydrate salt (needles) in a vacuum at -10o. [Ennor & Stocken Biochemical Preparations 5 9 1957, Biochem J 43 190 1958, Beilstein 4 III 1170, 4 IV 2425.]
