Description
Deoxyribonucleic acid (abbreviated DNA) is the molecule that carries genetic information for the development and functioning of an organism. DNA is made of two linked strands that wind around each other to resemble a twisted ladder — a shape known as a double helix. Each strand has a backbone of alternating sugar (deoxyribose) and phosphate groups. Attached to each sugar is one of four bases: adenine (A), cytosine (C), guanine (G), or thymine (T). The two strands are connected by chemical bonds between the bases: adenine bonds with thymine, and cytosine bonds with guanine. The sequence of the bases along DNA’s backbone encodes biological information, such as the instructions for making a protein or RNA molecule.
Chemical Properties
Lyophilized
History
Deoxyribonucleic Acid (DNA) was first recognized and identified by the Swiss biologist Johannes Friedrich Miescher in 1869 during his research on white blood cells. The double helix structure of a DNA molecule was later discovered through experimental data by James Watson and Francis Crick. Finally, it was proved that DNA is responsible for storing genetic information in living organisms.
Uses
Carboxy-terminal FLAG-BAP
? Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).
Uses
Salmon DNA, useful for biochemical research.
Uses
DNA (freeze-dried)(pure biological)(pure vegetal)(deoxyribonucleic acid) is a surface film-forming protein with moisturizing action. DnA’s large macromolecules do not enable it to penetrate the skin. In addition, its affinity with the stratum corneum layer keeps it anchored to the skin’s surface, where it serves to protect and retain skin moisture. Although the use of DnA was particularly popular in the 1980s, its incorporation into cosmetic products since then has practically disappeared. It is usually used in a potassium salt form obtained from fish sperm.
Biological Functions
DNA is the genetic material which car-ries all the hereditary information. Genes are small segments of DNA, consisting mostly of 250 – 2 million base pairs. A gene code for a polypeptide molecule, where three nitrogenous bases sequence stands for one amino acid. Polypeptide chains are further folded in secondary, tertiary and quaternary structures to form different proteins. As every organism contains many genes in its DNA, different types of proteins can be formed. Proteins are the main functional and structural molecules in most organisms. Apart from storing genetic information, DNA is involved in:
Replication process: Transferring the genetic information from one cell to its daughters and from one generation to the next and equal distribution of DNA during the cell division
Mutations: The changes which occur in the DNA sequences
Transcription
Cellular Metabolism
DNA Fingerprinting
Gene Therapy
General Description
Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).
Biochem/physiol Actions
The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.
