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Fluorescein diacetate Structure

Fluorescein diacetate

Chemical Properties

Melting point 200-203 °C(lit.)
Boiling point 494.73°C (rough estimate)
Density  1.2738 (rough estimate)
refractive index  1.6810 (estimate)
storage temp.  −20°C
solubility  acetone: soluble25mg/mL
form  Crystalline Powder
pka 6.4(at 25℃)
color  Light yellow
PH Range Weak green uorescence (6.0) to strong green uorescence (7.2)
Water Solubility  Soluble in chloroform, DMSO, ethanol, and water.
λmax 490nm
BRN  62575
Major Application LED devices, petroleum markers, identifying cancerous cells, detecting bacteria, virus, mitochondrial function in neurons, target molecules, living cells, viable Giardia cysts in water samples, apoptosis, evaluating live/dead state of algae, measurement of totalmicrobial activity in soil, treating ischemia
Biological Applications Assessing membrane integrity/permeability;43–50 detecting/counting microorganisms; measuring microbial activity; as a substrate for measuring acylases activity, amidases activity,aminopeptidases activity, arylsulfatases activity,chymotrypsin activity, esterases activity;galactosidases activity, glucosidases activity, lipases activity, phosphatases activity, proteases activity; cell viability assay; cytotoxicity assay; implantable medical devices; as temperature sensor; treating ischemic disease
CAS DataBase Reference 596-09-8(CAS DataBase Reference)
NCI Dictionary of Cancer Terms FDA
FDA UNII YL39R93PRE
EPA Substance Registry System Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)- (596-09-8)

Safety

Symbol(GHS)
Signal wordWarning
Hazard statements H315-H319
Precautionary statements P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  24/25-36/37/39-27-26
WGK Germany  3
TSCA  Yes
HS Code  32129000
NFPA 704:
0
1 0

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Fluorescein diacetate Chemical Properties,Usage,Production

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