Description
6-Aminofluorescein (6-AF) is a new fluorescence dye with bright colors and high fluorescence intensity. Its chemical structure history contains a nitrogen group and a fluorescent group. The presence of the fluorescent component enables 6-Aminofluorescein to emit a distinct fluorescent signal when excited, making it valuable for fluorescent labeling and imaging. It is widely used in biological science research, chemical analysis and fluorescence microscopy.
Biological Functions
6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed "buckysomes". 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein. 6-Aminofluorescein has been used to determine its ability to potentiate the antiviral activity of poly r (A-U).
References
[1] BHARATE G Y, QIN H, FANG J. Poly(Styrene-Co-Maleic Acid)-Conjugated 6-Aminofluorescein and Rhodamine Micelle as Macromolecular Fluorescent Probes for Micro-Tumors Detection and Imaging[J]. Journal of Personalized Medicine, 2022, 12. DOI:10.3390/jpm12101650.
[2] Müller, Werner EG, and Heinz Christoph Schr?der, eds. Biological Response Modifiers—Interferons, Double-Stranded RNA and 2′, 5′-Oligoadenylates. Vol. 14. Springer Science & Business Media, 2012.
