EC 1.14.14.3

Uses

Luciferase has been used as a component in Mix B respiration medium for the determination of the ATP synthetic activity of the H⁺-ATP synthase. It has also been used in thermal aggregation to assay CnoX (YbbN) activity.

Uses

Luciferase may be used:

• to treat mammary tumor cryosections for bioluminescence imaging studies
• as a component of luciferase reaction mixture
• in bioluminescence assay membrane preparations of Zymomonas mobilis

Uses

Luciferase from Vibrio fischeri has been used in a study to assess kinetics of light emission and oxygen consumption by bioluminescent bacteria. It has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.

General Description

Alkanal reduced FMN-oxygen oxidoreductase (1-hydroxylating, luminescing)

Biochem/physiol Actions

Luciferase catalyzes the conversion of reduced flavin mononucleotide in the presence of oxygen and long-chain aldehyde to flavin mononucleotide, visible light and a fatty acid. It is a reporter enzyme for sensitive quantitation. The lucifearse fusion proteins have been useful in many protein based interaction studies especially in living cells.