Uses
Luciferase has been used as a component in Mix B respiration medium for the determination of the ATP synthetic activity of the H
+-ATP synthase. It has also been used in thermal aggregation to assay CnoX (YbbN) activity.
Uses
Luciferase may be used:
- to treat mammary tumor cryosections for bioluminescence imaging studies
- as a component of luciferase reaction mixture
- in bioluminescence assay membrane preparations of Zymomonas mobilis
Uses
Luciferase from
Vibrio fischeri has been used in a study to assess kinetics of light emission and oxygen consumption by bioluminescent bacteria. It has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.
General Description
Alkanal reduced FMN-oxygen oxidoreductase (1-hydroxylating, luminescing)
Biochem/physiol Actions
Luciferase catalyzes the conversion of reduced flavin mononucleotide in the presence of oxygen and long-chain aldehyde to flavin mononucleotide, visible light and a fatty acid. It is a reporter enzyme for sensitive quantitation. The lucifearse fusion proteins have been useful in many protein based interaction studies especially in living cells.
