ChemicalBook CAS DataBase List 9014-00-0

9014-00-0

Name	EC 1.14.14.3
CAS	9014-00-0
EINECS(EC#)	232-751-7
Molecular Formula	NA
MDL Number	MFCD00131545

Synonyms

LUCIFERASE EC 1.14.14.3 Bacteria luciferase BACTERIAL LUCIFERASE LUCIFERASE, BACTERIAL Luciferase froM Bacteria Vibrio harveyi luciferase Vibrio fischeri luciferase Alkanal monooxygenase (FMN-linked) Photorhabdus luminescens luciferase LUCIFERASE FIREFLY RECOMBINANT 95+% Luciferase (Photobacterium leiognathi) Luciferase from Photobacterium fischeri Luciferase, bacterial from Vibrio harveyi LUCIFERASE BACTERIAL FROM VIBRIO*FISCHER I Native Photinus pyralis (firefly) Luciferase 1: PN: CN103239735 FIGURE: 4A claimed sequence luciferase from vibrio fischeri (photobacterium f) Native Vibrio fischeri (Photobacterium f) Luciferase LUCIFERASE FROM PH. FISCHERI PARTIALLY PURIFIED POWDER LUCIFERASE FROM PHOTOBACT. FISCHERI, LYO PHIL., POWDER Native Photobacterium phosphoreum (Lux) Bacterial Luciferase Bacterial Luciferase, Luciferase from Photobacterium fischeri

Chemical Properties

storage temp.	-20°C
form	powder
color	White to light yellow
Water Solubility	Water: 50 mg/mL
EPA Substance Registry System	Luciferase (9014-00-0)

Safety Data

WGK Germany	3
F	3-10-21
TSCA	TSCA listed
Storage Class	11 - Combustible Solids
Hazard Classifications	Resp. Sens. 1

Hazard Information

Uses

Luciferase from Vibrio fischeri has been used in a study to assess kinetics of light emission and oxygen consumption by bioluminescent bacteria. It has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.

Uses

Luciferase has been used as a component in Mix B respiration medium for the determination of the ATP synthetic activity of the H⁺-ATP synthase. It has also been used in thermal aggregation to assay CnoX (YbbN) activity.

Uses

Luciferase may be used:

to treat mammary tumor cryosections for bioluminescence imaging studies
as a component of luciferase reaction mixture
in bioluminescence assay membrane preparations of Zymomonas mobilis

General Description

Alkanal reduced FMN-oxygen oxidoreductase (1-hydroxylating, luminescing)

Biochem/physiol Actions

Luciferase catalyzes the conversion of reduced flavin mononucleotide in the presence of oxygen and long-chain aldehyde to flavin mononucleotide, visible light and a fatty acid. It is a reporter enzyme for sensitive quantitation. The lucifearse fusion proteins have been useful in many protein based interaction studies especially in living cells.

in vivo

Luciferase (labels HEK293 cells stably expressing pmeLUC；i.p. or intramass injection) can measure the ATP concentration in the tumor microenvironment in real-time in the mouse tumor model inoculated with HEK293 cells stably expressing pmeLUC^[1].
Luciferase can clearly monitor the growth of cancer cells in a mouse model in which RENCA^LUC cells are inoculated subcutaneously^[2].

storage

Store at -20°C

Supplier

Nanjing Dulai Biotechnology Co., Ltd.

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Shanghai Macklin Biochemical Co.,Ltd.

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Shanghai Baoman Biotechnology Co., Ltd.

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Sigma-Aldrich

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Beijing NuoqiYa Biotechnology Co., Ltd.

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Shanghai YuanYe Biotechnology Co., Ltd.

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Raw material medicin reagent co.,Ltd

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Hubei Hongxin Ruiyu Fine Chemical Co., Ltd.

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Guangzhou LES biological Technology Co.,Ltd.

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Shanghai Zhen Li Biological Technology Co., Ltd

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Hubei widely chemical technology Co., Ltd.

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