Synthesis
The protected amino acid derivative (2 mg) was placed in a 1.5 mL glass vial with a screw cap. The appropriate acid solution (1 mL) and 0.1 M HCl were added to the HFIP. The reaction mixture was incubated at room temperature for 240 min, and the product Fmoc-Lys-OH hydrochloride was obtained by RT-HPLC and MALDI-TOF or ESI HR mass spectrometry.
Description
Fmoc-Lys-OH hydrochloride is a cleavable ADC linker used to synthesize antibody-drug conjugates (ADCs). Fmoc-Lys-OH hydrochloride is also an alkyl chain-based PROTAC linker that can be used to synthesize PROTACs.
Chemical Properties
White solid
Uses
Fmoc-Lys-OH hydrochloride is used as an active pharmaceutical intermediate and in the synthesis of nε-(7-diethylaminocoumarin-3-carboxyl)- and nε-(7-methoxycoumarin-3-carboxyl)-l-fmoc lysine as tools for protease cleavage detection by fluorescence.
reaction suitability
reaction type: Fmoc solid-phase peptide synthesis
IC 50
Non-cleavable Linker
