O-6-Amino-6-deoxy-L-glycero-D-galacto-hepto-pyranosyliden-(1-2-3)o-beta-D-talopyranosyl-(1-5)-2-deoxy-N-methyl-D-streptamin

Bezeichnung:O-6-Amino-6-deoxy-L-glycero-D-galacto-hepto-pyranosyliden-(1-2-3)o-beta-D-talopyranosyl-(1-5)-2-deoxy-N-methyl-D-streptamin

CAS-Nr31282-04-9

Englisch Name:Hygromycin B

CBNumberCB9219683

SummenformelC20H37N3O13

Molgewicht527.52

MOL-Datei31282-04-9.mol

Synonyma

Hygromycin B

O-6-Amino-6-deoxy-L-glycero-D-galacto-hepto-pyranosyliden-(1-2-3)o-beta-D-talopyranosyl-(1-5)-2-deoxy-N-methyl-D-streptamin

O-6-Amino-6-deoxy-L-glycero-D-galacto-hepto-pyranosyliden-(1-2-3)o-beta-D-talopyranosyl-(1-5)-2-deoxy-N-methyl-D-streptamin physikalisch-chemischer Eigenschaften

Schmelzpunkt	160-180°C
alpha	D20 +20.2° (c = 1 in water)
Siedepunkt	608.13°C (rough estimate)
Dichte	1.4252 (rough estimate)
Brechungsindex	1.6300 (estimate)
storage temp.	2-8°C
Löslichkeit	H₂O: 50 mg/mL As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days.
Aggregatzustand	powder
pka	7.1, 8.8(at 25℃)
Farbe	faintly brown to brown
PH	pH (25g/l, 25℃ ) : 9.5～12.5
Wasserlöslichkeit	Miscible with water, methanol, buffer solution and ethanol. Immiscible with less polar solvents.
Merck	14,4852
BRN	6755837
Stabilität	Hygroscopic
EPA chemische Informationen	Hygromycin B (31282-04-9)

Sicherheit

Kennzeichnung gefährlicher	T+,Xn,T
R-Sätze:	26/27/28-41-42/43-40-36-23/24/25
S-Sätze:	23-26-28-36/37/39-45-22-36-36/37
RIDADR	UN 3462 6.1/PG 1
WGK Germany	3
RTECS-Nr.	WK2130000
F	19
HazardClass	6.1
PackingGroup	I
HS Code	29419059
Toxizität	mmo-esc 250 mg/L AACHAX 18,798,80

Gefahreninformationscode (GHS)

Hygromycin B Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R26/27/28:Sehr giftig beim Einatmen, Verschlucken und Berührung mit der Haut.
R41:Gefahr ernster Augenschäden.
R42/43:Sensibilisierung durch Einatmen und Hautkontakt möglich.
R40:Verdacht auf krebserzeugende Wirkung.
S-Sätze Betriebsanweisung:
S23:Gas/Rauch/Dampf/Aerosol nicht einatmen(geeignete Bezeichnung(en) vom Hersteller anzugeben).
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S28:Bei Berührung mit der Haut sofort abwaschen mit viel . . . (vom Hersteller anzugeben).
S36/37/39:Bei der Arbeit geeignete Schutzkleidung,Schutzhandschuhe und Schutzbrille/Gesichtsschutz tragen.
S45:Bei Unfall oder Unwohlsein sofort Arzt zuziehen (wenn möglich, dieses Etikett vorzeigen).
S22:Staub nicht einatmen.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
Beschreibung
Hygromycin B is an aminoglycoside antibiotic that has been found in S. hygroscopicus. It is active against E. coli (MIC = 150 μg/ml) and cytotoxic to both prokaryotic and eukaryotic cells via inhibition of protein synthesis. Hygromycin B has commonly been used in molecular and cell biology applications to select for transformed cells expressing the E. coli hygromycin resistance genes hyg or hph.
Chemische Eigenschaften
White to off-white lyophilized powder
Verwenden
Hygromycin B, a selective antibiotic that is effective on most bacteria, fungi and higher eukaryotes, inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome
Verwenden
antibacterial, anthelmintic; LD50 ip (rat) 63mg/Kg
Verwenden
Hygromycin B is a trisaccharide aminoglycoside with a terminal cyclitol produced by Streptomyces hygroscopicus, reported by researchers at Eli Lily in the early 1950s. Hygromycin B belongs to the destomycin family of aminoglycosides which contain a unique cyclic structure linking the terminal saccharide. Hygromycin B exhibits broad spectrum antibiotic and anthelmintic activity, and is used as a feed additive for swine and poultry. Hygromycin B inhibits polypeptide synthesis by binding peptidyl-tRNA and preventing translocation by Elongation Factor 2.
Verwenden
Hygromycin B is an aminoglycoside and a selective antibiotic agent for the hph gene. Hygromycin B inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S rib osome
Application
Hygromycin B was found in the culture broth of Streptomyces hygroscopicus by Lilly Research Laboratories in 1953, as a mixture with hygromycin A. It showed activity against a variety of gram-positive and gram-negative bacteria as well as fungi. Hygromycin B has been used for therapy of helminthic infections in swine and poultry.
Allgemeine Beschreibung
Amorphous solid or tan powder.
Air & Water Reaktionen
Water soluble.
Reaktivität anzeigen
Weakly basic.
Brandgefahr
Flash point data for Hygromycin B are not available; however Hygromycin B is probably combustible.
Biochem/physiol Actions
Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation. Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.
Sicherheitsprofil
Poison by intraperitoneal route. Mutation data reported. When heated to decomposition it emits toxic fumes of Nox
Lager
+4°C
Einzelnachweise
[1] brodersen de, clemons wm jr, carter ap, et al. the structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin b on the 30s ribosomal subunit. cell, 2000, 103 (7): 1143-1154.
[2] ganoza mc, kiel mc. a ribosomal atpase is a target for hygromycin b inhibition on escherichia coli ribosomes. antimicrob agents chemother, 2001, 45 (10): 2813-2819.

Hygromycin B Upstream-Materialien And Downstream Produkte

O-6-Amino-6-deoxy-L-glycero-D-galacto-hepto-pyranosyliden-(1-2-3)o-beta-D-talopyranosyl-(1-5)-2-deoxy-N-methyl-D-streptamin Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

31282-04-9, Hygromycin B Verwandte Suche:

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