N-(Tri(hydroxymethyl)methyl)glycin

Bezeichnung:N-(Tri(hydroxymethyl)methyl)glycin

CAS-Nr5704-04-1

Englisch Name:Tricine

CBNumberCB5853856

SummenformelC6H13NO5

Molgewicht179.17

MOL-Datei5704-04-1.mol

Synonyma

N-(Tri(hydroxymethyl)methyl)glycin

N-(Tri(hydroxymethyl)methyl)glycin physikalisch-chemischer Eigenschaften

Schmelzpunkt	186-188 °C (dec.)
Siedepunkt	311.69°C (rough estimate)
Dichte	1.05 g/mL at 20 °C
Dampfdruck	0.002-0.004Pa at 20-25℃
Brechungsindex	1.4240 (estimate)
storage temp.	Keep in dark place,Inert atmosphere,Room temperature
Löslichkeit	H₂O: 4 M at 20 °C, clear, colorless
Aggregatzustand	crystalline
Farbe	clear colorless (40 % (w/w) in H₂O) solution
PH	4.0-6.0 (1M in H2O)
pka	8.1(at 25℃)
Säure-Base-Indikators(pH-Indikatoren)	7.4 - 8.8
Geruch (Odor)	Odorless
Wasserlöslichkeit	soluble
Merck	14,9651
BRN	1937804
Stabilität	Stable.
LogP	-2.87 at 25℃
CAS Datenbank	5704-04-1(CAS DataBase Reference)
EPA chemische Informationen	Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)

Sicherheit

Kennzeichnung gefährlicher	Xi
R-Sätze:	36/37/38
S-Sätze:	26-36-36/37/39
WGK Germany	3
F	3
TSCA	Yes
HS Code	29052900

Gefahreninformationscode (GHS)

Tricine Chemische Eigenschaften,Einsatz,Produktion Methoden

R-Sätze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S36/37/39:Bei der Arbeit geeignete Schutzkleidung,Schutzhandschuhe und Schutzbrille/Gesichtsschutz tragen.
Chemische Eigenschaften
White/clear crystalline powder
Verwenden
Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets. It is a zwitterionic amino acid commonly useful in the pH range of 7.4-8.8 and the pKa is 8.05 at 25°C. This buffer is also used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).
Definition
ChEBI: N-tris(hydroxymethyl)methylglycine is a Good's buffer substance, pKa = 8.15 at 20 ℃. It is functionally related to a member of tris and a glycine. It is a tautomer of a N-tris(hydroxymethyl)methylammonioacetate.
Allgemeine Beschreibung
Tricine and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood.
Biologische Aktivität
Electrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.
läuterung methode
Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]

Tricine Upstream-Materialien And Downstream Produkte

N-(Tri(hydroxymethyl)methyl)glycin Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

5704-04-1, Tricine Verwandte Suche:

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