补骨脂定

生物活性靶点体外研究体内研究补骨脂定试剂级价格

补骨脂定

CAS号:18642-23-4
英文名:psoralidin
中文名:补骨脂定
CBNumber:CB41384552
分子式:C20H16O5
分子量:336.34
MOL File:18642-23-4.mol

补骨脂定化学性质

熔点 :290-292°
沸点 :458.8±34.0 °C(Predicted)
密度 :1.383±0.06 g/cm3(Predicted)
储存条件 :Keep in dark place,Inert atmosphere,Store in freezer, under -20°C
溶解度 :DMSO: soluble5mg/mL, clear (warmed)
形态 :powder
酸度系数(pKa) :8.70±0.20(Predicted)
颜色 :white to beige
InChIKey :YABIJLLNNFURIJ-UHFFFAOYSA-N

安全信息

WGK Germany :3
海关编码 :2932.20.4500

补骨脂定性质、用途与生产工艺

生物活性 Psoralidin 是从 Psoralea corylifolia 的种子中分离出来的，是 COX-2 和 5-LOX 的双重抑制剂。具有抗癌，抗菌和抗炎作用。Psoralidin 显著下调 NOTCH1 信号传导。Psoralidin 还极大地诱导 ROS 产生。
靶点

COX-2

5-LOX

体外研究

Three breast cancer cell (BCC)populations (ALDH ^- cells, ALDH ⁺ cells, and commercial BSCSs) are sensitive to Psoralidin treatment (10, 15, 20, and 25 μM; 24 hours) with IC ₅₀ s ranging from 18 to 21 μM; however, the MCF-12A cells were resistant to Psoralidin.
Psoralidin (30 μM; 24 hours) results in a significant induction of apoptosis for ALDH ^- cells, ALDH ⁺ cells, and commercial BCSCs.
Psoralidin treatment also downregulates NOTCH1 expression in both ALDH ^- and ALDH ⁺ cells.

Cell Viability Assay

Cell Line:	ALDH ^- cells, ALDH ⁺ cells, commercial breast cancer stem cells (BSCSs), and normal breast epithelial cells (MCF-12A)
Concentration:	10, 15, 50, and 25 μM
Incubation Time:	24 hours
Result:	IC ₅₀ s of 18 to 21 μM for ALDH ^- cells, ALDH ⁺ cells, commercial BCSCs.

Apoptosis Analysis

Cell Line:	ALDH ^- cells, ALDH ⁺ cells, and commercial BCSCs
Concentration:	20 and 30 μM
Incubation Time:	24 hours
Result:	No significant induction of apoptosis was observed for any of the three cell types following treatment at 20 μM. However, 53.60%, 44.1%, and 45.9% of cells were apoptotic at 30 μMin ALDH ^- cells, ALDH ⁺ cells, and commercial BCSCs, respectively.

体内研究

Psoralidin (5 mg/kg) regulates expression of pro-inflammatory cytokines that play an important role in inflammatory diseasesin IR-irradiated lung of BALB/c mouse.

Animal Model:	BALB/c mice
Dosage:	5 mg/kg
Administration:	Intraperitoneal application; 30 min before and 1 h after IR irradiation (20 Gy).
Result:	Anti-inflammatory effect in IR-irradiated mice.