右布洛芬

生物活性靶点体外研究体内研究右布洛芬试剂级价格

右布洛芬

CAS号:51146-56-6
英文名:(S)-(+)-Ibuprofen
中文名:右布洛芬
CBNumber:CB00131404
分子式:C13H18O2
分子量:206.28
MOL File:51146-56-6.mol

右布洛芬化学性质

熔点 :49-53 °C(lit.)
比旋光度 :57 º (c=2, EtOH)
沸点 :285.14°C (rough estimate)
密度 :1.0364 (rough estimate)
折射率 :59 ° (C=2, EtOH)
闪点 :>230 °F
储存条件 :Sealed in dry,Room Temperature
溶解度 :45% (w/v) aq 2-hydroxypropyl-β-cyclodextrin: 1.5 mg/mL
酸度系数(pKa) :4.41±0.10(Predicted)
形态 :solid
颜色 :white
旋光性 (optical activity) :[α]20/D +59°, c = 2 in ethanol
水溶解性 :insoluble
BRN :3590022
稳定性 :Stable. Incompatible with strong oxidizing agents.
InChIKey :HEFNNWSXXWATRW-JTQLQIEISA-N
CAS 数据库 :51146-56-6(CAS DataBase Reference)

安全信息

危险品标志 :Xn
危险类别码 :63-22
安全说明 :45-36/37
WGK Germany :3
海关编码 :29163990

右布洛芬性质、用途与生产工艺

生物活性 (S)-(+)-Ibuprofen ((S)-Ibuprofen)，一种 Ibuprofen 的 S(+)-对映异构体，是一种有效的 COX-1 和 COX-2 抑制剂，IC50 分别为 2.1μM 和 1.6 μM。(S)-(+)-Ibuprofen 具有止痛，抗炎，抗癌和退热作用。
靶点

COX-1

2.1 μM (IC ₅₀ )

COX-2

1.6 μM (IC ₅₀ )

体外研究

(S)-(+)-Ibuprofen (HCT-15 and HCA-7 cells; 0-1000 µM; 8 days) treatment reduces concentration dependently cell survival in both cell lines to a similar extent.
(S)-(+)-Ibuprofen (HCT-15 and HCA-7 cells; 0-1000 µM; 20-72 hours) treatment causes a G0/G1 phase block as well as apoptosis.
(S)-(+)-Ibuprofen (HCT-15 and HCA-7 cells; 900 µM; 4-72 hours) treatment shows a down regulation of cyclin A and B and an increase of the cell cycle inhibitory protein p27Kip-1.
(S)-(+)-Ibuprofen inhibits COX activity, thromboxane formation, and platelet aggregation.
(S)-(+)-Ibuprofen inhibits the activation of NF-κB in response to T-cell stimulation with an IC ₅₀ of 61.7 μM.

Cell Proliferation Assay

Cell Line:	HCT-15 and HCA-7 cells
Concentration:	0 µM, 200 µM, 400 µM, 600 µM, 700 µM, 800 µM, 900 µM, and 1000 µM
Incubation Time:	8 days
Result:	Reduced concentration dependently cell survival in both cell lines to a similar extent.

Cell Cycle Analysis

Cell Line:	HCT-15 and HCA-7 cells
Concentration:	0 µM, 200 µM, 400 µM, 600 µM, 800 µM, 900 µM, and 1000 µM
Incubation Time:	24 hours (HCT-15) or 20 hours (HCA-7)
Result:	Caused a G0/G1 phase block.

Apoptosis Analysis

Cell Line:	HCT-15 and HCA-7 cells
Concentration:	0 µM, 200 µM, 400 µM, 600 µM, 800 µM, 900 µM, and 1000 µM
Incubation Time:	72 hours
Result:	Induced cell apoptosis.

Western Blot Analysis

Cell Line:	HCT-15 and HCA-7 cells
Concentration:	900 µM
Incubation Time:	4 hours, 8 hours, 16 hours, 24 hours, 32 hours, 48 hours and 72 hours
Result:	Decreased levels of Cyclin D1 protein.

体内研究

(S)-(+)-Ibuprofen (15 mg/kg/day; intraperitoneal injection; five days a week; for 4 weeks) treatment inhibits tumor growth of HCA-7 and HCT-15 xenografts in the nude mice model.

Animal Model:	NMRI (nu/nu) male mice (6-8 week old ) injected with HCA-7 and HCT-15 cells
Dosage:	15 mg/kg/day
Administration:	Intraperitoneal injection; five days a week; for 4 weeks
Result:	Inhibited tumor growth of HCA-7 and HCT-15 xenografts in mice.