ChemicalBook CAS DataBase List 75277-39-3

75277-39-3

Name	HEPES sodium salt
CAS	75277-39-3
EINECS(EC#)	278-169-7
Molecular Formula	C8H17N2NaO4S
MDL Number	MFCD00036463
Molecular Weight	260.29
MOL File	75277-39-3.mol

Synonyms

2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID SODIUM SALT 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID SODIUM SALT 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID, DISODIUM SALT 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANE-SULFONIC ACID HALF-SODIUM SALT 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID HEMISODIUM SALT 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID LOWSODIUM 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID, SODIUM SALT 4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID HEMISODIUM SALT 4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID SODIUM SALT 4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID SODIUM SALT BUFFER HEPES (HEPES)2-NA HEPES, HALF-SODIUM SALT HEPES HEMISODIUM SALT HEPES LOWSODIUM HEPES NA HEPES NA-SALT HEPES, SODIUM HEPES SODIUM SALT HEPES SODIUM SALT BUFFER

Chemical Properties

Appearance	white powder
Melting point	234 °C
density	1.504[at 20℃]
vapor pressure	0Pa at 25℃
storage temp.	Store at RT.
solubility	H₂O: 1 M at 20 °C, clear, colorless
form	dry powder
color	White
PH	10.0-12.0 (1M in H2O)
PH Range	6.8 - 8.2
pka	7.5(at 25℃)
Stability:	Stable. Hygroscopic-protect from moisture. Incompatible with strong oxidizing agents.
biological source	synthetic
Water Solubility	It is soluble in water.
Sensitive	Hygroscopic
InChI	1S/C8H18N2O4S.Na.H2O/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;;/h11H,1-8H2,(H,12,13,14);;1H2/q;+1;/p-1
InChIKey	ZNJHFNUEQDVFCJ-UHFFFAOYSA-M
SMILES	O.[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
CAS DataBase Reference	75277-39-3(CAS DataBase Reference)
EPA Substance Registry System	75277-39-3(EPA Substance)
Absorption	≤0.2 at 260 in H2O at 1M ≤0.2 at 280 in H2O at 1M

Safety Data

Symbol(GHS)	GHS07
Signal word	Warning
Hazard statements	H315-H319-H335
Precautionary statements	P280-P304+P340-P305+P351+P338
Safety Statements	S22:Do not breathe dust . S24/25:Avoid contact with skin and eyes . less more
WGK Germany	3
F	3-10
TSCA	Yes
REACH Registrations	Active
HS Code	29335995
Storage Class	13 - Non Combustible Solids

Hazard Information

Chemical Properties

white powder

Uses

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Definition

ChEBI: Sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate is an organic sodium salt, a N-(2-hydroxyethyl)piperazine and a N-(sulfoalkyl)piperazine. It contains a 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate.

General Description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Flammability and Explosibility

Notclassified

Biological Activity

Multi purpose buffer used in biological research.

Synthesis

103-76-4

1562-00-1

75277-39-3

The general procedure for the synthesis of N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt from N-hydroxyethylpiperazine and sodium 2-hydroxyethanesulfonate is as follows: magnetic stirring in a 70-mL fluoropolymer-lined steel autoclave equipped with internal thermocouples for monitoring the temperature of the liquid phase, a pressure gauge, a pressure-reducing valve, and an exhaust valve. First, amine feedstock, anhydrous or aqueous sodium 2-hydroxyethanesulfonate solution, and anhydrous or aqueous sodium hydroxide solution were added to the open-topped autoclave. Subsequently, the autoclave was assembled and immersed in an electrically heated oil bath. The stirred reaction mixture was rapidly heated to the specified reaction temperature with the exhaust valve closed and maintained at that temperature for the time specified in Examples 9, 10 and 13. Upon completion of the reaction, the mixture was cooled to room temperature, the reactor was opened, and the reaction product was dissolved in a sufficient amount of water to ensure complete dissolution of all solids.

storage

Room temperature

References

[1] Patent: US2006/89509, 2006, A1. Location in patent: Page/Page column 4-6
[2] Patent: US2006/89509, 2006, A1. Location in patent: Page/Page column 4-6

Material Safety Data Sheet(MSDS)

Spectrum Detail

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