ChemicalBook CAS DataBase List 7365-45-9

7365-45-9

Name	HEPES
CAS	7365-45-9
EINECS(EC#)	230-907-9
Molecular Formula	C8H18N2O4S
MDL Number	MFCD00006158
Molecular Weight	238.3
MOL File	7365-45-9.mol

Synonyms

2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID 4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID GOOD'S BUFFER HEPES HEPES-ACID HEPES BUFFER HEPES BUFFERED SALINE HEPES BUFFER PH 7.5 HEPES BUFFER PH 8.0 LABOTEST-BB LT00138109 LABOTEST-BB LT00233156 N-(2-HYDROXYETHYL)PIPERAZINE N-2-ETHANESULFONIC ACID N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANESULFONIC ACID N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANE SULPHONIC ACID 4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci hepesfreeacid N-(2-Hydroxyethyl)piper 4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid

Chemical Properties

Appearance	White crystalline powder
Melting point	234-238 °C
Boiling point	408℃[at 101 325 Pa]
bulk density	560kg/m3
density	1.07 g/mL at 20 °C
vapor pressure	0Pa at 25℃
refractive index	n20/D 1.339
storage temp.	2-8°C
solubility	H₂O: 1 M at 20 °C, clear, colorless
form	powder
color	White
PH	5.0-6.5 (1Maqueous solution)
PH Range	7.0 - 7.6
pka	7.5(at 25℃)
Stability:	Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
Water Solubility	Soluble
λmax	λ: 260 nm Amax: 0.06 λ: 280 nm Amax: 0.04
Detection Methods	T,NMR
Merck	14,4654
BRN	883043
InChIKey	JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP	-3.85 at 20℃
CAS DataBase Reference	7365-45-9(CAS DataBase Reference)
EPA Substance Registry System	7365-45-9(EPA Substance)

Safety Data

Hazard Codes	Xi
Risk Statements	R36/37/38:Irritating to eyes, respiratory system and skin . less more
Safety Statements	S24/25:Avoid contact with skin and eyes . S22:Do not breathe dust . S36:Wear suitable protective clothing . S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . less more
WGK Germany	1
RTECS	TL6809000
TSCA	Yes
HS Code	29335995

Hazard Information

Description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Chemical Properties

White crystalline powder

Uses

Biological buffer.

Uses

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

Definition

ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.

General Description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Biological Activity

Multi purpose buffer used in biological research.

storage

Room temperature

Purification Methods

Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]

Material Safety Data Sheet(MSDS)

Spectrum Detail

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