ChemicalBook CAS DataBase List 7365-45-9

7365-45-9

Name	HEPES
CAS	7365-45-9
EINECS(EC#)	230-907-9
Molecular Formula	C8H18N2O4S
MDL Number	MFCD00006158
Molecular Weight	238.3
MOL File	7365-45-9.mol

Synonyms

2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID 4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID GOOD'S BUFFER HEPES HEPES-ACID HEPES BUFFER HEPES BUFFERED SALINE HEPES BUFFER PH 7.5 HEPES BUFFER PH 8.0 LABOTEST-BB LT00138109 LABOTEST-BB LT00233156 N-(2-HYDROXYETHYL)PIPERAZINE N-2-ETHANESULFONIC ACID N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANESULFONIC ACID N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANE SULPHONIC ACID 4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci hepesfreeacid N-(2-Hydroxyethyl)piper 4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid

Chemical Properties

Appearance	White crystalline powder
Melting point	234-238 °C
Boiling point	408℃[at 101 325 Pa]
density	1.07 g/mL at 20 °C
vapor pressure	0Pa at 25℃
refractive index	n20/D 1.339
storage temp.	2-8°C
solubility	H₂O: 1 M at 20 °C, clear, colorless
form	powder
color	White
PH	5.0-6.5 (1Maqueous solution)
PH Range	7.0 - 7.6
pka	7.5(at 25℃)
Stability:	Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
Water Solubility	Soluble
λmax	λ: 260 nm Amax: 0.06 λ: 280 nm Amax: 0.04
Detection Methods	T,NMR
Merck	14,4654
BRN	883043
InChIKey	JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP	-3.85 at 20℃
CAS DataBase Reference	7365-45-9(CAS DataBase Reference)
EPA Substance Registry System	7365-45-9(EPA Substance)

Safety Data

Hazard Codes	Xi
Risk Statements	R36/37/38:Irritating to eyes, respiratory system and skin . less more
Safety Statements	S24/25:Avoid contact with skin and eyes . S22:Do not breathe dust . S36:Wear suitable protective clothing . S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . less more
WGK Germany	1
RTECS	TL6809000
TSCA	Yes
HS Code	29335995

Hazard Information

Description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Chemical Properties

White crystalline powder

Uses

Biological buffer.

Uses

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

Definition

ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.

General Description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Biological Activity

Multi purpose buffer used in biological research.

storage

Room temperature

Purification Methods

Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]

Material Safety Data Sheet(MSDS)

Spectrum Detail

Well-known Reagent Company Product Information

Supplier

Hubei xinghengkang Chemical Technology Co., Ltd

Telephone027-88878869 18162384755

Websitehttp://www.xhkhgkj.com/

Shanghai Jizhi Biochemical Technology Co. Ltd.

Telephone400-400-400-9004166 18616739031

Websitehttp://www.acmec-e.com/

Nanjing Search Biotech Co., Ltd

Telephone+86-025-81682922 +86-13515111588

Websitehttp://www.searchbio.com.cn/

AILY INTL CHEMICAL CORP LTD

Telephone025-83208295 18913388297

Websitehttps://www.chemicalbook.com/ShowSupplierProductsList15679/0.htm

Shandong Boyi Biotechnology Co. LTD

Telephone0516-18118583671 18118583671

Websitehttps://www.boyishengwu.cn

Hubei New Desheng Materials Technology Co., Ltd.

Telephone 18971041571

Websitehttp://www.whdsbio.com

Suzhou yacoo science co.,Ltd

Telephone0512-87182056 13451648594

Websitehttp://www.yacoo.com.cn

WuHan Difu Biotechnology Co.Ltd

Telephone 18008681304

Websitehttps://www.chemicalbook.com/supplier/25292897/

Hangzhou Bingochem Co., Ltd.

Telephone0571-87632989

Websitehttp://www.bingochem.com/

Hunan Hengfei bio-pharmaceutical Co. , Ltd.

Telephone0746-6222194 13874779848

Websitehttps://www.chemicalbook.com/ShowSupplierProductsList1856679/0.htm

J & K SCIENTIFIC LTD.

Telephone010-82848833 400-666-7788

Websitehttp://www.jkchemical.com

Meryer (Shanghai) Chemical Technology Co., Ltd.

Telephone4006608290; 18621169109

Websitehttps://www.meryer.com/cn/index/

3B Pharmachem (Wuhan) International Co.,Ltd.

Telephone821-50328103-801 18930552037

Websitehttps://www.chemicalbook.com/ShowSupplierProductsList13285/0.htm

future industrial shanghai co., ltd

Telephone400-0066400 13621662912

Websitehttp://www.jonln.com

Alfa Aesar

Telephone400-6106006

Websitehttp://chemicals.thermofisher.cn

TCI (Shanghai) Development Co., Ltd.

Telephone021-67121386

Websitehttps://www.tcichemicals.com/CN/zh/

BeiJing Hwrk Chemicals Limted

Telephone0757-86329057 18501085097

Websitehttp://www.hwrkchemical.com

Energy Chemical

Telephone021-021-58432009 400-005-6266

Websitehttp://www.energy-chemical.com

1of4

Related Products

Send Inquriy