Chemical Properties
White Solid
Uses
One of the most biologically studied of the primary prostaglandins. Closely related to Prostaglandin E2 (PGE2) in that both
prostaglandins are biosynthesized from the same precursors and that PGF2 is
the synthetic reduction product of PGE2. Phospholipase A2 (PLA2) is a key enzyme for biosynthesis of PGF2α.
Oxytocic; abortifacient.
Brand name
Prostin F2 Alpha (Pharmacia
& Upjohn).
Description
Dinoprost tromethamine is a synthetic analogue of prostaglandin. F2α is an autocrine hormone present in many mammalian tissues. It is used to cause luteolysis in the cow, sow and mare. It is given as a single injection by im or sc routes, the recommended dosage rate is 25 mg cow; 10 mg sow and 5 mg mare. It is rapidly absorbed from the injection site. It has an extremely short half life of only a few minutes, it is almost completely cleared following one or two passages through the liver and/or lungs.
Originator
Prostin F2A,Upjohn,UK,1972
Definition
dinoprost tromethamine is a synthetic analogue of the naturally occurring prostaglandin F2 alpha. Prostaglandin F2 alpha stimulates myometrial activity, relaxes the cervix, inhibits corpus luteal steroidogenesis, and induces luteolysis by direct action on the corpus luteum.
Indications
Dinoprost tromethamine salt (Prostaglandin F2α tromethamine salt) is an orally active, potent prostaglandin F (PGF) receptor (FP receptor) agonist. Dinoprost tromethamine salt is a luteolytic hormone produced locally in the endometrial luminal epithelium and corpus luteum (CL). Dinoprost tromethamine salt plays a key role in the onset and progression of labour.
Manufacturing Process
A solution of tris(hydroxymethyl)aminomethane (1.645 grams) in 3.0 ml of
water at 60°C is added with vigorous stirring to a solution of PGF2α, (5.00
grams) in 700 ml of acetonitrile which has just been
ought to its boiling
point. The vessel which contained the aqueous amine solution is rinsed with
three 0.66 ml portions of water, each rinsing being added with vigorous
stirring to the acetonitrile solution. The mixture is then cooled to 25°C by
immersion of the vessel in cool water. At the cloud point, the vessel wall
(glass) below the liquid surface is scratched vigorously with a glass rod. The
mixture is then maintained at 25°C for 24 hours.
The resulting crystals are collected by filtration under nitrogen, washed on the
filter with 50 ml of acetonitrile, and then dried by passing nitrogen at 50°C
through the filter cake for one hour. Drying is completed in an oven at 70°C
for 8 hours to give 5.965 grams of the tris(hydroxymethyl)aminomethane salt of PGF2α in free flowing crystalline form; MP 100°-101°C.
Therapeutic Function
Smooth muscle stimulant
Biochem/physiol Actions
Prostaglandin F2α is induced by uterine-produced oxytocin and acts on the corpus luteum to cause luteolysis and inhibit progesterone production.
