1045-69-8

Testosterone acetate (Item No. 26915) is an analytical reference standard categorized as an anabolic androgenic steroid. It is an ester of the naturally occurring androgen, testosterone (Item Nos. 15645 | ISO60154). Testosterone acetate is regulated as a Schedule III compound in the United States. This product is intended for research and forensic applications.

White Solid

An Androgen. Controlled substance.

ChEBI: An androstanoid that is the acetate derivative of testosterone.

A solution of 4.23 g (14.7 mmol) of testosterone in 8 ml of pyridine dried over potassium hydroxide overnight (5 g of KOH to 250 ml of pyridine) is prepared in a 250-ml flask equipped with magnetic stirring. The solution is cooled to 0° C. with a water/ice mixture. 4 ml of acetic anhydride is added to it dropwise. The reaction mixture is left to return to room temperature, and the solution is stirred overnight at RT. After the starting product is no longer found in analytical TLC, 30 ml of distilled water and 30 ml of ethyl acetate are poured into the flask, and the mixture is stirred. The phases are separated with a separating funnel, and the aqueous phase is extracted with 3×20 ml of ethyl acetate. The organic phases are combined, and the solution obtained is washed with 20 nil of distilled water, dried over anhydrous magnesium sulfate, and evaporated in a rotary evaporator. The product thus obtained is dried under reduced pressure using a vacuum pump for 4 hours to remove all the solvents. 4.8 g (yield=99.0%) of testosterone acetate is obtained.

Testosterone acetate is an androgen ester and an intermediate in the synthesis of testosterone from progesterone, isolated from microorganisms.