Нетральн. красный

Химические свойства

dark green powder

Использование

Neutral Red is a common histology stain used to determine cell viability. It is also used as indicator for nitrogen determination; pH indicator; Dyes and metabolites.

прикладной

Transition of pH Indicator: pH 6.8 - 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it.

Определение

ChEBI: Neutral red is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+).

Подготовка

(a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture.

Общее описание

Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.

Staining Procedures

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

Методы очистки

In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]