БЕТА-АМИЛАЗА

Использование

β-Amylase is a gel filtration molecular weight marker that can be used in gel filtration chromatography and protein chromatography. β-Amylase can be purified by affinity precipitation with alginate.

Общее описание

β-Amylases belongs to the class of amylolytic enzymes.

Биохимия/физиол Действия

β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. Pure, crystalline β-amylase preparation consists of four isoenzymes with different isoelectric points. The enzyme polymerizes very rapidly through the sulfhydryl groups in the absence of reducing agents. p-Chloromercuribenzoate inhibits the polymerization and the enzymatic activity. The reducing agents mercaptoethanol or dithiothreitol can completely restore the activity.