重组胰蛋白酶

Recombinant trypsin
9002-07-7

￥218.00 10MG 起订

上海更新日期：2021-05-11

上海雅心生物技术有限公司

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产品详情:

中文名称：: 重组胰蛋白酶

英文名称：: Recombinant trypsin

CAS号：: 9002-07-7

纯度规格：: ≥95%

产品类别：: 蛋白酶重组蛋白酶

产品编号：: RPT0201

产品等级：: 医药级

规格：: 10mg、100mg、1g

月产量：: 1000000000

Recombinant Trypsin

Catalog Number: RPT0201

CAS: 9002-07-7
EC: 3.4.21.4

Storage temperature: 2-8℃

Source: Recombinant trypsin is a genetically engineered protein expressed in E.coli

Protein Sequence:The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin

1.DESCRIPTION

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.

2.MAIN FEATURES

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Source

Recombinant E. coli

Appearance

White or off white, or yellowish powder

Specific activity

≥3800 USP units/mg pro.

Purity(SDS-PAGE)

Single major band

Molecular Weight(SDS-PAGE)

24.0±2.4 kDa

UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A₂₅₃of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

3.RECOMMEND USAGE

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant trypsin lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

5.PRODUCT ADVANTAGE

l Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.

l Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

l High purity: Higher specific activity.Host protein residues is les

Recombinant trypsin expressed in E.coli.

公司简介

上海雅心生物技术有限公司主要从事基因工程蛋白酶的研发和生产，目前的主要品种为基因工程羧肽酶B、基因工程胰蛋白酶和基因工程脂肪酶A；为本公司自主研发，拥有自主知识产权。公司拥有自组研发团队，拥有世界先进的重组生物技术；自主研发的重组生物用酶为世界上生物用酶的首创！基因工程羧肽酶B，基因工程胰蛋白酶和基因工程脂肪酶A都是由基因工程方法生产，纯度高，不含杂酶，无羧肽酶A、糜蛋白酶等其他蛋白酶活性；无动物源性，出口产品无需进行终产品的动物源性检验。制品中不含蛋白酶抑制剂，产品安全。公司秉承“科技致胜”的理念，坚持“提供高纯度重组蛋白酶”，并愿与广大同行交流，有需求的客户提供优质的服务！产品经多

成立日期	(17年)
注册资本	100.0万人民币
员工人数	10-50人
年营业额	￥ 100万-300万
经营模式	贸易,工厂,试剂,定制,服务
主营行业	色谱分析,质谱,医药中间体,生化试剂