佛波醇12-十四酸酯13-乙酸酯|TQ0198

Phorbol 12-myristate 13-acetate
16561-29-8

￥436 1mg 起订

￥1050 5mg 起订

￥1770 10mg 起订

上海更新日期：2024-12-02

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产品详情:

中文名称：: 佛波醇12-十四酸酯13-乙酸酯

英文名称：: Phorbol 12-myristate 13-acetate

CAS号：: 16561-29-8

品牌：: TargetMol

产地：: 美国

保存条件：: keep away from direct sunlight,store under nitrogen,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.5%

产品类别：: 抑制剂

货号：: TQ0198

Product Introduction

Bioactivity

名称	Phorbol 12-myristate 13-acetate
描述	Phorbol 12-myristate 13-acetate (PMA) belongs to the phorbol ester group of natural products and is an activator of PKC, SphK, and NF-κB. Phorbol 12-myristate 13-acetate induces THP1 cell differentiation.
细胞实验	αT3-1 and LβT-2 cells are grown in monolayer cultured in DMEM in humidified incubator 5% CO2 at 37°C. Serum starvation is with 0.1% FCS in the same medium for 16 h. GnRH and PMA are then added for the length of time as indicated. In general, αT3-1 cells are transiently transfected by ExGen 500 or by jetPRIME, while LβT2 cells only by jetPRIME transfection reagent. For experiments with dominant-negative (DN) PKCs, αT3-1 cells (in 6 cm plates) are transfected with 1.5 μg of p38α-GFP with 3 μg of control vector, pCDNA3, or with 3 μg of the DN-PKCs constructs. For LβT2 cells, transfections are performed (in 10 cm plates) with 4 μg of p38α-GFP along with 9 μg of control vector, pCDNA3, or with 9 μg of the DN-PKCs constructs. Approximately 30 h after transfection, the cells are serum-starved (0.1% FCS) for 16 h and later stimulated with GnRH or PMA, washed twice with ice-cold PBS, treated with the lysis buffer, followed by one freeze-thaw cycle. Cells are harvested; following centrifugation (15,000×g, 15 min, 4°C) supernatants are taken for immunoprecipitation experiments [2].
动物实验	All experiments are performed with male Wistar rats (weighing 250-280 g). One hundred and thirty-five Wistar rats are randomly divided into seven groups. (1) Rats in the sham group (n=21) are given a lateral cerebral ventricle injection of 0.9% normal saline; (2) Rats in the IR group (n=21) are given a lateral cerebral ventricle injection of 0.9% normal saline 30 min before middle cerebral artery occlusion (MCAO); (3) Rats in the Carbenoxolone (CBX) group (n=21) are given a lateral cerebral ventricle injection of CBX (5 μg/mL×10 μL) 30 min before MCAO; (4) Rats in the Sch-6783 group (n=21) are given a lateral cerebral ventricle injection of DZX (2 mM×30 μL) 30 min prior to MCAO; (5) Rats in the 5-HD group (n=21) are given a lateral cerebral ventricle injection of 5-HD (100 mM×10 μL), and after 10 min, DZX is injected 15 min prior to MCAO; (6) The rats in the DZX + Ro group (n=15) are given a lateral cerebral ventricle injection of DZX, and after 10 min, Ro-31-8425 (400 μg/kg) is injected 15 min prior to MCAO; (7) The rats in the 5-HD+PMA group (n=15) are given an intraperitoneal injection of PMA (200 μg/kg) after the injection of 5-HD and DZX [3].
体外活性	方法：球体培养的人类黑色素瘤细胞 WM 系列用 Phorbol 12-myristate 13-acetate (50 ng/mL) 处理 3 天，使用 MTS 方法检测细胞生长情况。结果：Phorbol 12-myristate 13-acetate 促进黑色素瘤细胞增殖，WM35 细胞的细胞数提高到 265%。[1] 方法：人单核白血球细胞 THP-1 用 Phorbol 12-myristate 13-acetate (200 ng/mL) 处理 1-5 天，使用光学显微镜评估形态，使用 Flow Cytometry 方法检测靶点表达。结果：Phorbol 12-myristate 13-acetate 诱导 THP-1 细胞分化为巨噬细胞样细胞 (THP-1 巨噬细胞)，CD11 和 CD14 的细胞表面表达增加。[2] 方法：人静脉内皮细胞 HUVECs 用 Phorbol 12-myristate 13-acetate (10-40 ng/mL) 处理 8 h，使用 Wound healing migration assay 检测细胞迁移情况。结果：短期 Phorbol 12-myristate 13-acetate 处理可增强内皮细胞迁移。[3]
体内活性	方法：为研究佛波酯对啮齿类动物大脑发育的影响，将 Phorbol 12-myristate 13-acetate (100-500 μg/kg) 单次腹腔注射给药给缺乏 IL-18 或 IRAK-4 的新生大鼠和小鼠，24 h、7 天或14 天后处死动物。结果：Phorbol 12-myristate 13-acetate 在大脑中诱导炎症反应并引起广泛的神经退行性变。缺乏 IL-18 或 IRAK-4 对 Phorbol 12-myristate 13-acetate 诱导的脑损伤有保护作用。[4] 方法：为构建急性小鼠耳炎症模型，将 Phorbol 12-myristate 13-acetate (20 μL 125 μg/mL PMA 丙酮溶液) 局部处理 CD-1 小鼠双耳，风干并完全吸收。结果：用 Phorbol 12-myristate 13-acetate 攻击的耳组织在施用约 2 小时开始出现炎症迹象，包括肿胀和发红。[5]
存储条件	keep away from direct sunlight,store under nitrogen,store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 60 mg/mL (97.27 mM) H2O : Insoluble
关键字	PKC \| SphK \| Nuclear factor-κB \| Protein kinase C \| Inhibitor \| Phorbol 12 myristate 13 acetate \| TPA \| inhibit \| Phorbol 12myristate 13acetate \| Phorbol myristate \| Nuclear factor-kappaB \| Sphingosine kinase \| Phorbol 12-myristate \| NF-κB \| Phorbol 12-myristate 13-acetate
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佛波醇12-十四酸酯13-乙酸酯|||PMA|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

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