佛波醇12-十四酸酯13-乙酸酯
佛波醇12-十四酸酯13-乙酸酯 性质
| 熔点 | 65-79oC |
|---|---|
| 沸点 | 571.87°C (rough estimate) |
| 密度 | 1.17±0.1 g/cm3 (20 ºC 760 Torr) |
| 折射率 | 1.4900 (estimate) |
| 储存条件 | -20°C |
| 溶解度 | 可溶于DMSO，DMSO 溶液可以在20℃下避光保存至少六个月。 |
| 酸度系数(pKa) | 11.19±0.70(Predicted) |
| 形态 | 精细结晶粉末 |
| 颜色 | 灰白色至浅黄色 |
| 水溶解性 | Very soluble in methanol, soluble in DMSO, chloroform, ethanol or acetone, ether, ethyl acetate, DMF or acetonitrile. Insoluble in water. |
| 敏感性 | Light Sensitive |
| BRN | 2407201 |
| 稳定性 | 可在-20°下的DMSO或乙醇溶液保存长达2个月。 |
| InChIKey | PHEDXBVPIONUQT-RGYGYFBISA-N |
| SMILES | CCCCCCCCCCCCCC(=O)O[C@@H]1[C@@H](C)[C@@]2(O)C(C=C(CO)C[C@@]3(O)C2C=C(C)C3=O)C4C(C)(C)[C@]14OC(C)=O |
| CAS 数据库 | 16561-29-8(CAS DataBase Reference) |
| EPA化学物质信息 | 12-O-Tetradecanoylphorbol 13-acetate (16561-29-8) |
佛波醇12-十四酸酯13-乙酸酯 用途与合成方法
|
PKC 11.7 nM (EC 50 ) |
In order to examine the role of PKC in p38MAPK phosphorylation, the cells are stimulated with the PKC activator, PMA (100 nM), which mimics the binding of DAG, the natural activator of PKC, to the C1 region of the PKCs. p38MAPK phosphorylation by PMA is observed in the two cell types similar to that observed by GnRH in αT3-1 cells, that is, a slow sustained activation (3.2-fold and 3.6-fold, respectively at 30 min). The paradoxical findings that PKCs activated by GnRH and PMA play a differential role in p38MAPK phosphorylation may be explained by differential localization of the PKCs. Basal, GnRH- and PMA- stimulation of p38MAPK phosphorylation in αT3-1 cells is mediated by Ca
2+
influx via voltage-gated Ca
2+
channels and Ca
2+
mobilization, while in the differentiated LβT2 gonadotrope cells it is mediated only by Ca
2+
mobilization.
THP-1 cells are differentiated into macrophage-like cells (THP-1 macrophages) by incubation in the presence of PMA (200 ng/mL; 1-5 days), which leads to a macrophage-like phenotype characterized by changes in morphology and increased cell surface expression of CD11 and CD14.
In the monocytic cell line THP-1, PMA results in a more differentiated phenotype than VD3, according to adherence, loss of proliferation, phagocytosis of latex beads, and expression of CD11b and CD14.
PMA is a PKC agonist, which reverses the damage induced by 5-hydroxydecanoic acid (5-HD). Thus, activation of the mitoKATP protected mitochondrial function in SOD and MDA via the PKC pathway.
安全信息
| 危险品标志 | Xi |
|---|---|
| 危险类别码 | 38-36/37/38 |
| 安全说明 | 36/37-26 |
| 危险品运输编号 | 2928 |
| WGK Germany | 3 |
| RTECS号 | QH4377000 |
| F | 8-10 |
| 危险等级 | 6.1(a) |
| 包装类别 | II |
| 海关编码 | 29153900 |
| 存储类别 | 6.1A - 可燃，急性毒性物质（类别1和类别2） 剧毒的危险物质 |
| 危险性类别 | 急性毒性 类别1 经皮 急性毒性 类别1 吸入 急性毒性 类别2 经口 致癌性 类别2 严重眼损伤 类别1 呼吸道致敏物 类别1 皮肤腐蚀 类别1B 皮肤致敏物 类别1 |
| 毒害物质数据 | 16561-29-8(Hazardous Substances Data) |
| 毒性 | Diesters of phorbol, particularly 12-O-tetradecanoylphorbol 13-acetate, also known as phorbol myristate acetate, have been identified as the potent, highly lipophilic tumor-promoting components in croton oil. They are potent promoters of skin tumors in vivo and have been shown to transform cultured fibroblasts and embryonic cells previously exposed to polycyclic aromatic hydrocarbon carcinogens in vitro. The structure of phorbol, the parent alcohol is shown below. Phorbol esters also have important effects on leukocytes and on the immune system of the intact animal. There appears to be a specific receptor for phorbol esters on T cells, a finding that may account for the selective toxicity of phorbol esters for T cells. The mechanism of tumor promotion by phorbol esters is still largely unknown, but findings suggest an initial cell membrane interaction (often with stimulation of second messenger synthesis and increased protein kinase activity), with subsequent effects on nuclear regulatory events. |
佛波醇12-十四酸酯13-乙酸酯 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2026-09-15 | HY-18739R | 佛波醇12-十四酸酯13-乙酸酯 | 16561-29-8 | 1 mg | 1650 |
| 2026-09-15 | HY-18739R | 佛波醇12-十四酸酯13-乙酸酯 | 16561-29-8 | 5 mg | 3300 |
