1. Extraction process: kelp powder → leaching → centrifugation → taking supernatant → low temperature evaporation and concentration to 1/4 of the original volume → 95% ethanol precipitation → ether degreasing → acetone decolorization → absolute ethanol washing → vacuum freeze drying → Kombu Crude Polysaccharide.
2. Purification method: add water to dissolve kelp crude polysaccharide, and adjust the pH value to 8 with ammonia water; under the condition that the temperature is lower than 50 ℃, add 20% H2O2 dropwise until the solution is pale yellow, and then keep it for 2 hours; Distilled water was dialyzed for 3 d. After 3 d, the non-permeable part was removed by Sevag method; then dialyzed with distilled water for 3 d, added 3 times the volume of 95% ethanol for precipitation, and left standing at 4 °C overnight; centrifuged the next day, and collected the precipitate. Preliminary purified white fibrous kelp polysaccharide can be obtained by vacuum freeze-drying.
3. Extraction and purification: Weigh kelp coarse powder and place it in a round-bottomed flask, add petroleum ether (60-90°C) to soak for 2 hours; reflux extraction for 1 hour, filter; evaporate the filter residue to dry the solvent, and extract twice with 95% ethanol under reflux , filtered for 1 h each time; the filter residue was placed in a round-bottomed flask, soaked in distilled water for 1 h, refluxed for 2 h, and filtered; then distilled water was added for reflux extraction for 2 h, filtered; the filtrates were combined, concentrated to an appropriate amount, and the protein was removed by the Sevage method. 95% ethanol was added to make the alcohol content 80%, left standing overnight, and suction filtered; the filter residue was washed several times with absolute ethanol and acetone in turn to obtain the refined polysaccharide.
Laminarin mainly includes brown algae (such as sodium alginate, also known as alginate, the content in kelp is about 19.7%), fucoidan (also known as laminarin, seaweed sulfate polysaccharide, fucoidan, the content in kelp is about 1%), Fucoidan (also known as fucoidan, the main component is fucoidan, also known as fucoidan) and other 3 types, consisting of β(1-3)-glucan and some β(1-6)- Composed of glycosidic bonds, it is a kind of neutral glucan, widely present in brown algae, and also the main chemical component in kelp of the family Pterophyceaceae or kelp of the kelp family. It has the functions of regulating immunity, anti-tumor, anti-coagulation, regulating blood lipids. , hypoglycemic, anti-radiation, antioxidant, antibacterial, antiviral and other pharmacological effects, but also has various effects such as lowering blood pressure, fatigue resistance, anti-pulmonary fibrosis, regulating tyrosine kinase activity, and anti-mutation.
The dry crude polysaccharide product is a yellow-brown lumpy solid with a fishy smell of sea. Decolorized by H2O2 and freeze-dried to obtain a white fibrous solid, soluble in water, easily soluble in hot water, insoluble in acid and alkali, insoluble in high-concentration ethanol, methanol, acetone and other organic solvents, non-volatile, pH 6.5 , the specific rotation is [α]D20=-1.25°(c=0.80, H2O).
Kombu, also known as kelp, seaweed, sea kelp, sea cabbage. Extracted from the thallus of Laminaria japonica Aresch. and Ecklonia kurome Okam.
Laminarin is a storage glucan and is now used to study its interaction with laminarinase as its substrate.
Laminarin obtained from Laminaria digitata. It is a .beta.-(1-3)-linked D-glucan with .BETA.-(1-6) linkages. The major M-series contains 20-30 glucosyl residues linked to terminal mannitol, and a minor G-series with 22-28 glucosyl residues. There is a 3 to 1 ratio of M-series to G-series molecules. There is an average of 1.3 branches per molecule.
The fronds of Laminaria and Saccharina species contain laminarin, a low molecular weight storage glucan. It is localized to the vacuoles. Laminarin structure contains β-(1,6)-intrachain linked (1,3)-β-d-glucan.
Laminarin is a polysaccharide of glucose found in brown algae and utilized as a food reserve.
