3,5-Dinitrosalicylic acid appears as yellow, flaky crystals with a melting point of 173–174 °C. It is soluble in alcohol, benzene, and ether but only slightly soluble in water.
3,5-Dinitrosalicylic acid is used in the quantification of reducing sugars by colorimetry. It is utilized for the analysis of glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. It is also used to detect reducing substances in urine and for quantifying carbohydrate level in blood. It acts as an important raw material and an intermediate in the production of organic chemicals such as agrochemicals, organic synthesis, pharmaceuticals and dyes.
3,5-Dinitrosalicylic acid was used as a reagent for the preparation of oxazolines from amino alcohols and for the spectrophotometric determination of ampicillin. It was also used to measure the effects of silver nanoparticles on the membrane leakage of the reducing sugars.
3,5-Dinitrosalicylic acid is a salicylic acid derivative used in colorimeteric determination of reducing sugars.
3,5-Dinitrosalicylic acid is prepared by the nitration of salicylic acid. Salicylic acid is added to a mixed acid solution and nitrated at 15–25°C. The reaction mixture is then slowly poured into ice water, yielding a yellow solid precipitate. After filtration and washing with water, the pure product is obtained.
ChEBI: A monohydroxybenzoic acid consisting of 2-hydroxybenzoic acid having nitro substituents at the 3- and 5-positions. It is used in colorimetric testing for the presence of free carbonyl groups (C2O) in reducing sugars.
3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.
Crystallise the acid from H2O. [Beilstein 10 IV 270.]
