Casein-fluorescein isothiocyanate type I

Casein fluorescein isothiocyanate from bovine milk has been used:

• in accessing zona pellucida (ZP) and perivitelline space (PVS) to evaluate changes induced by protease treatment
• to make a reference line in immunochromatographic devices
• in running gel for the documentation of proteinases

Casein is a major milk phospho-protein and constitutes about 80% of the total protein content in milk. Bovine milk is composed of four types of casein such as, α_S1, α_S2, β, and κ. Casein exists as micelles in milk.

Casein micelle system is involved in the prevention of pathological calcification of mammary glands.