Propidium iodide

CAS25535-16-4

MFC27H34IN4+

MW541.5

EINECS247-081-0

Chemical Properties

Melting point	220-225 °C (dec.) (lit.)
Density	1.5072 (estimate)
storage temp.	2-8°C
solubility	Difficult to mix.
form	Flaky Powder
color	Dark red
Water Solubility	Soluble in water at 1mg/ml
λmax	493 nm, 535 nm
Sensitive	Light Sensitive & Hygroscopic
BRN	3843838
Biological Applications	Detecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens,cancer
Major Application	Electroluminescent displays; photoresists
CAS DataBase Reference	25535-16-4(CAS DataBase Reference)

Safety Information

Hazard Codes	Xi,Xn
Risk Statements	36/37/38-68-67-48/20/22-40-36/38-22-20-63
Safety Statements	26-36-22-36/37
WGK Germany	3
RTECS	SF7949600
F	3-8-10-23
HS Code	29339900

MSDS

Usage And Synthesis

Description

Propidium iodide (PI) is membrane impermeant and generally excluded from viable cells. However, it can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. It binds to DNA and RNA by intercalating between the bases. PI is excited at 488-535 nm with an emission maximum of 617 nm. This probe is suitable for fluorescence microscopy and flow cytometry.
Chemical Properties

Dark Red Solid
Uses

Propidium Iodide is a phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes. Propidium iodide may be used in flow cytometry to evaluate cell viab ility when used with other dyes that stain viable cells or cells that are early in the apoptosis process.
Uses

Used as fluorescent stain for nucleic acids,and used in flow cytometry to evaluate cell viability
Uses

Antibacterial;Detergent
Uses

1 mg/mL in water
Uses

Propidium iodide is the most common red-fluorescent nuclear stain. It can also be excited using excitation filters appropriate for green-fluorescent dyes.
General Description

Extinction coefficient(493 nm): 5900 M ^-1cm^-1;(287 nm): 55,700 M ^-1cm^-1;
Biochem/physiol Actions

Primary TargetMembrane impermeable DNA intercalator
Purification Methods

It crystallises as red crystals from H2O containing a little KI. It fluoresces strongly with nucleic acids. [Watkins J Chem Soc 3064 1952, Beilstein 22 III/IV 5519.] TOXIC.

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