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Propidium iodide

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Propidium iodide Basic information
Propidium iodide Chemical Properties
  • Melting point:220-225 °C (dec.)(lit.)
  • Density 1.5072 (estimate)
  • storage temp. 2-8°C
  • solubility Difficult to mix.
  • form Flaky Powder
  • color Dark red
  • Water Solubility Soluble in water at 1mg/ml
  • λmax493 nm, 535 nm
  • Sensitive Light Sensitive & Hygroscopic
  • BRN 3843838
  • Biological ApplicationsDetecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens,cancer
  • Major ApplicationElectroluminescent displays; photoresists
  • CAS DataBase Reference25535-16-4(CAS DataBase Reference)
Safety Information
Propidium iodide Usage And Synthesis
  • Chemical PropertiesDark Red Solid
  • Uses1 mg/mL in water
  • UsesPropidium iodide is the most common red-fluorescent nuclear stain. It can also be excited using excitation filters appropriate for green-fluorescent dyes.
  • UsesAntibacterial;Detergent
  • UsesPropidium Iodide is a phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes. Propidium iodide may be used in flow cytometry to evaluate cell viab ility when used with other dyes that stain viable cells or cells that are early in the apoptosis process.
  • UsesUsed as fluorescent stain for nucleic acids,and used in flow cytometry to evaluate cell viability
  • Purification MethodsIt crystallises as red crystals from H2O containing a little KI. It fluoresces strongly with nucleic acids. [Watkins J Chem Soc 3064 1952, Beilstein 22 III/IV 5519.] TOXIC.
Propidium iodide(25535-16-4)Related Product Information
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