Propidium iodide CAS#: 25535-16-4

Propidium iodide

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Propidium iodide Basic information

Propidium iodide Chemical Properties

Melting point:220-225 °C (dec.)(lit.)
Density 1.5072 (estimate)
storage temp. 2-8°C
solubility Difficult to mix.
form Flaky Powder
color Dark red
Water Solubility Soluble in water at 1mg/ml
λmax493 nm, 535 nm
Sensitive Light Sensitive & Hygroscopic
BRN 3843838
Biological ApplicationsDetecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens,cancer
Major ApplicationElectroluminescent displays; photoresists
CAS DataBase Reference25535-16-4(CAS DataBase Reference)

Safety Information

MSDS

Propidium iodide Usage And Synthesis

Chemical PropertiesDark Red Solid
Uses1 mg/mL in water
UsesPropidium iodide is the most common red-fluorescent nuclear stain. It can also be excited using excitation filters appropriate for green-fluorescent dyes.
UsesAntibacterial;Detergent
UsesPropidium Iodide is a phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes. Propidium iodide may be used in flow cytometry to evaluate cell viab ility when used with other dyes that stain viable cells or cells that are early in the apoptosis process.
UsesUsed as fluorescent stain for nucleic acids,and used in flow cytometry to evaluate cell viability
Purification MethodsIt crystallises as red crystals from H2O containing a little KI. It fluoresces strongly with nucleic acids. [Watkins J Chem Soc 3064 1952, Beilstein 22 III/IV 5519.] TOXIC.

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