Histone H3K27Me1 (21-44)-GK-biotin (trifluoroacetate salt)

Histone H3K27Me1 (21-44)-GK-biotin is a peptide fragment of histone H3 that corresponds to amino acid residues 22-45 of the human histone H3.1 and H3.2 sequences. It is monomethylated at lysine 27 and biotinylated via a C-terminal GK linker. Monomethylation of histone H3 at lysine 27 is associated with actively transcribed genes and positively correlates with H3K36 trimethylation.1 Levels of H3K27Me1 are increased in tumor tissue isolated from patients with metastatic hormone-naïve and castration-resistant prostate cancer.2 Histone H3K27Me1 (21-44)-GK-biotin has been used in a high-throughput screen for inhibitors of the histone demethylase jumonji AT-rich interactive domain 1B (JARID1B).3WARNING This product is not for human or veterinary use.

[1] KARIN J FERRARI. Polycomb-dependent H3K27me1 and H3K27me2 regulate active transcription and enhancer fidelity.[J]. Molecular Cell, 2014, 53 1: 49-62. DOI: 10.1016/j.molcel.2013.10.030
[2] JÖRG ELLINGER. Global histone H3K27 methylation levels are different in localized and metastatic prostate cancer.[J]. Cancer Investigation, 2012, 30 2: 92-97. DOI: 10.3109/07357907.2011.636117
[3] JOYCE SAYEGH. Identification of small molecule inhibitors of Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase by a sensitive high throughput screen.[J]. The Journal of Biological Chemistry, 2013, 288 13: 9408-9417. DOI: 10.1074/jbc.m112.419861