Histone H3K27Me2 (21-44)-GK-biotin (trifluoroacetate salt)

Histone H3K27Me2 (21-44)-GK-biotin is a peptide fragment of histone H3 that corresponds to amino acid residues 22-45 of the human histone H3.1 and 3.2 sequences. It is dimethylated at lysine 27 and biotinylated via a C-terminal GK linker. Dimethylation of H3K27 is present on large chromatin domains across approximately 70% of total histone H3 and prevents firing of non-cell type-specific enhancers.1 H3K27Me2 accumulates in gene bodies of genes with low levels of expression in mouse embryonic stem cells. Histone H3K27Me2 (21-44)-GK-biotin has been used as a positive control in a high-throughput screen for inhibitors of the histone demethylase jumonji AT-rich interactive domain 1B (JARID1B).2WARNING This product is not for human or veterinary use.

[1] KARIN J FERRARI. Polycomb-dependent H3K27me1 and H3K27me2 regulate active transcription and enhancer fidelity.[J]. Molecular Cell, 2014, 53 1: 49-62. DOI: 10.1016/j.molcel.2013.10.030
[2] JOYCE SAYEGH. Identification of small molecule inhibitors of Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase by a sensitive high throughput screen.[J]. The Journal of Biological Chemistry, 2013, 288 13: 9408-9417. DOI: 10.1074/jbc.m112.419861