Methyl 7-hydroxycoumarin-4-acetate

Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate, a natural coumarins derivative, is exploited for the synthesis of the switchable fluorescent substrates to be used in bacterial enzyme detection[1][2][3].

[1] Nascimento JSD, et al. Mapping the Biotransformation of Coumarins through Filamentous Fungi. Molecules. 2019 Sep 29;24(19). DOI:10.3390/molecules24193531
[2] Lizzul-Jurse A, et al. Readily functionalizable phosphonium-tagged fluorescent coumarins for enhanced detection of conjugates by mass spectrometry. Org Biomol Chem. 2016 Aug 10;14(32):7777-91. DOI:10.1039/c6ob01080f
[3] Giovannini G, et al. Coumarin-based, switchable fluorescent substrates for enzymatic bacterial detection. Talanta. 2018 Oct 1;188:448-453. DOI:10.1016/j.talanta.2018.06.018