Boc-Val-Leu-Lys-AMC acetate

Boc-Val-Leu-Lys-AMC is a sensitive, fluorogenic, and specific substrate of plasmin, as well as acrosin from the ascidian Halocynthia roretzi, porcine calpain isozymes I and II, and papain[1][2][3][4].

[1] T Sasaki, et al. Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates. J Biol Chem. 1984 Oct 25;259(20):12489-94. PMID:6092335
[2] R E Smith, et al. Direct photometric or fluorometric assay of proteinases using substrates containing 7-amino-4-trifluoromethylcoumarin. Thromb Res. 1980 Feb;17(3-4):393-402. DOI:10.1016/0049-3848(80)90074-2
[3] H Kato, et al. New fluorogenic peptide substrates for plasmin. J Biochem. 1980 Jul;88(1):183-90. PMID:6447693