Fmoc-PEG12-NHS ester is a PEG linker containing an Fmoc-protected amine and an NHS ester. The hydrophilic PEG spacer increases solubility in aqueous media. The Fmoc group can be deprotected under basic condition to obtain the free amine which can be used for further conjugations. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
Fmoc-PEG12-NHS ester (CAS 2227246-92-4) is a high-purity heterobifunctional PROTAC linker. Its synonyms include: FmocNH-PEG12-NHS ester, Fmoc-N-amido-PEG12-NHS ester, FmocNH-PEG12-CH₂CH₂COONHS, Fmoc-NH-PEG12-CH₂CH₂COONHS ester, and 2,5-Dioxopyrrolidin-1 -yl 1-(9H-fluoren-9-yl)-3-oxo-2,7,10,13,16,19,22,25,28,31,34,37,40-tridecaoxa-4-azatritetracontan-43-oate, amongst others. This compound contains functional groups comprising Fmoc-protected amines and NHS esters, whilst the hydrophilic polyethylene glycol (PEG) spacer improves its water solubility. It is widely used in site-specific biocoupling, the synthesis of PROTACs, and the modification of proteins or oligonucleotides.
