红斑红素 性质
| 熔点 | 156.5-157.0 °C (decomp)(Solv: ethyl ether (60-29-7)) |
|---|---|
| 沸点 | 638.8±55.0 °C(Predicted) |
| 密度 | 1.23±0.1 g/cm3(Predicted) |
| 形态 | 固体 |
| 颜色 | 黄至橙色 |
| InChI | InChI=1S/C21H22O5/c1-4-6-7-9-17(22)18-16-11-13-10-14(8-5-2)25-12-15(13)19(23)21(16,3)26-20(18)24/h5,8,10-12H,4,6-7,9H2,1-3H3/b8-5+/t21-/m1/s1 |
| InChIKey | SULYDLFVUNXAMP-WKOQKXSESA-N |
| SMILES | C1(/C=C/C)OC=C2C(=O)[C@]3(C)OC(=O)C(C(=O)CCCCC)=C3C=C2C=1 |
红斑红素 用途与合成方法
Rubropunctatin (1.5-30 μM; 24 h) shows selective tumoricidal effect on the human gastric carcinoma BGC-823 cells and no significant toxicity toward normal epithelial cell GES-1.
Rubropunctatin (5-30 μM; 6-24 h) induces apoptosis in a dose- and time-dependent manner in BGC-823 cells.
Rubropunctatin (0.75-8.0 µg/ml) exhibits DDPH radical scavenging activity, inhibition of super oxide radical generation and ferric reducing activity.
Cell Viability Assay
| Cell Line: | BGC-823 and GES-1cells |
| Concentration: | 1.5, 3, 6, 12, 15, 18, 30 μM |
| Incubation Time: | 24 hours |
| Result: |
Decreased the viability of BGC-823 cells with an IC
50
of 12.57 μM for 24 h.
Did not show obvious cytotoxic effects on the normal cells. |
Cell Cycle Analysis
| Cell Line: | BGC-823 cells |
| Concentration: | 0, 5, 10, 30 μM |
| Incubation Time: | 0, 6, 12, 24 hours |
| Result: |
Increased the percentage of cells in sub-G1 phase in a dose- and time-dependent manner.
|
Rubropunctatin (8-32 mg/kg; i.v. for 5 times) has anti-tumor effect mice.
Rubropunctamine inhibits 12-
O
-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice, with an ID
50
of 0.11 mg/ear.
| Animal Model: | Male nude mice (5 weeks) are inoculated with BGC-823 cells |
| Dosage: | 8, 32 mg/kg |
| Administration: | I.v. five times (day 1st, 4th, 7th, 10th, and 13th) |
| Result: |
Diminished the tumor volume by 11.1% (8 mg/kg) and 24.2% (32 mg/kg).
Reduced the tumor weight by 23.5% (8 mg/kg) and 37.7% (32 mg/kg). No significant difference was observed on the body weight. |
