2',7'-Dichlorofluorescein diacetate is a cell-permeable fluorogenic probe for detecting reactive oxygen species (ROS) and nitric oxide (NO).
off-white powder or crystals
2′,7′-Dichlorofluorescein diacetate may be used as a superior gas phase alternative to underivatized fluorescein and as a component/substrate of the 2′,7′-dichlorofluorescein diacetate assay to quantitate reactive oxygen species (ROS).
Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalyzed cleavage of the diacetate groups, DCDHF is oxidized by peroxynitrite yielding the highly fluorescent product
Excitation / Emission Maximum
492 nm/515 nm
