BCMA抗体 用途与合成方法
使用与人 TNFRSF17/BCMA 蛋白 Leu115 周围残基相对应的合成肽对动物进行免疫接种来产生多克隆抗体。使用蛋白质 A 和肽亲和色谱对抗体进行纯化。
TNFRSF17/BCMA Antibody 可识别内源水平的 TNFRSF17/BCMA 总蛋白。
(cellsignal-#47988)
使用 TNFRSF17/BCMA Antibody（上图）或 β-Actin (D6A8) Rabbit mAb #8457对 U266, H929 和 CCRF-CEM 细胞提取物进行蛋白质印迹分析。如预期所示，未在 CCRF-CEM 细胞中检测到 TNFRSF17/BCMA 蛋白。
W-Western IP-免疫沉淀法 IHC-免疫组织化学法 ChIP-染色质免疫沉淀法 IF-免疫荧光法 F-流式细胞术 E-P-ELISA 肽
B 细胞成熟抗原 (BCMA/TNFRSF17/CD269) 是一种跨膜糖蛋白，并且是 TNFR 超家族的一员 (1)。BCMA 表达主要限于 B 细胞系。通过该受体进行的促活信号转导可在结合其配体（BAFF 和 APRIL）时调控 B 细胞成熟和浆细胞分化，从而在体液免疫中起到关键作用 (2-6)。BCMA 在许多 B 细胞恶性肿瘤中表达，它在恶性浆细胞的细胞表面选择性表达且表达升高，因此，BCMA 因作为治疗多发性骨髓瘤的新治疗靶标而备受关注 (7-10)。
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.
TNFRSF17 (AAH58291, 1 a.a. ~ 184 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Identification of single nucleotide polymorphisms in the TNFRSF17 gene and their association with gastrointestinal disorders. Chae SC, et al. Mol Cells, 2010 Jan. PMID 20016944.Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity. Hosgood HD 3rd, et al. Occup Environ Med, 2009 Dec. PMID 19773279.Gut-associated lymphoid tissue contains the molecular machinery to support T-cell-dependent and T-cell-independent class switch recombination. Barone F, et al. Mucosal Immunol, 2009 Nov. PMID 19741596.Local network topology in human protein interaction data predicts functional association. Li H, et al. PLoS One, 2009 Jul 29. PMID 19641626.Common variants at ten loci influence QT interval duration in the QTGEN Study. Newton-Cheh C, et al. Nat Genet, 2009 Apr. PMID 19305408.
Tumor necrosis factor receptor superfamily member 17, B-cell maturation protein, CD269, TNFRSF17, BCM, BCMA
