Bz-VGR-AMC (87779-49-5) may be used for measuring the trypsin-like activity of the 20S proteasome, in combination with Suc-LLVY-AMC (Cat.# 10-2971, for assaying its chymotrypsin-like activity) and Z-LLE-AMC, (for determining its PGPH activity). Fluorogenic substrate. Ex.: 380nm Em.: 460nm.
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2) Kim?et al. (2002),?Navigation inside a protease: substrate selection and product exit in tricorn protease from Thermoplasma acidophilum; J. Mol. Biol.,?324?1041
3) Dahlmann?et al. (2000),?Different proteasome subtypes in a single tissue exhibit different enzymatic properties; J. Mol. Biol.,?303?643
4) Lightcap?et al. (2000),?Proteasome inhibition measurements: clinical application; Clin. Chem.,?46?673
