BZ-VAL-GLY-ARG-AMC TRIFLUOROACETATE SALT

Description

Bz-VGR-AMC (87779-49-5) may be used for measuring the trypsin-like activity of the 20S proteasome, in combination with Suc-LLVY-AMC (Cat.# 10-2971, for assaying its chymotrypsin-like activity) and Z-LLE-AMC, (for determining its PGPH activity). Fluorogenic substrate. Ex.: 380nm Em.: 460nm.

Uses

Bz-Val-Gly-Arg-AMC is a bioactive compound used in assaying the degree of inhibition conferred by PS-341, a potent peptidylboronic acid with anticancer properties.

References

[1] FRANK GILLARDON. The 20S proteasome isolated from Alzheimer’s disease brain shows post-translational modifications but unchanged proteolytic activity[J]. Journal of Neurochemistry, 2007, 101 6: 1483-1490. DOI:10.1111/j.1471-4159.2006.04438.x
[2] JEONG-SUN KIM. Navigation Inside a Protease: Substrate Selection and Product Exit in the Tricorn Protease from Thermoplasma acidophilum[J]. Journal of Molecular Biology, 2002, 324 5: Pages 1041-1050. DOI:10.1016/s0022-2836(02)01153-1
[3] BURKHARDT DAHLMANN . Different proteasome subtypes in a single tissue exhibit different enzymatic properties1[J]. Journal of Molecular Biology, 2000, 303 5: Pages 643-653. DOI:10.1006/jmbi.2000.4185
[4] E S LIGHTCAP. Proteasome inhibition measurements: clinical application.[J]. Clinical chemistry, 2000, 46 5: 673-683.