5,5'-二硫双(2-硝基苯甲酸)
5,5'-二硫双(2-硝基苯甲酸) 性质
| 熔点 | 240-245 °C (dec.)(lit.) |
|---|---|
| 沸点 | 671.9±55.0 °C(Predicted) |
| 密度 | 1.67 |
| 折射率 | 1.6900 (estimate) |
| 储存条件 | Keep in dark place,Sealed in dry,Room Temperature |
| 酸度系数(pKa) | 1.57±0.25(Predicted) |
| 形态 | 黄色固体 |
| 颜色 | 淡黄色至浅黄色 |
| 水溶解性 | Soluble in water and ethanol. |
| Merck | 14,3551 |
| BRN | 1896821 |
| 稳定性 | 稳定的。与强碱、强氧化剂不相容。 |
| InChIKey | KIUMMUBSPKGMOY-UHFFFAOYSA-N |
| CAS 数据库 | 69-78-3(CAS DataBase Reference) |
| EPA化学物质信息 | Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3) |
5,5'-二硫双(2-硝基苯甲酸) 用途与合成方法
• 巯基测定 — 与游离（还原）巯基基团 (—SH) 定量反应，以生成可检测的 TNB 产物
• 比色检测 — 有色产物可在比色皿或微孔板中进行分光光度测量
• 成熟的分析方法 — 可利用消光系数计算或通过与半胱氨酸标准进行比较，对肽或蛋白巯基基团进行定量测定
DTNB (Ellman’s Reag, Ellmans Reagenz, 5,5′-Dithiobis(2-nitrobenzoic acid), 5,5′-Dithiobis-2-nitrobenzoesäure)是一种非荧光探针，可用于定量样品中巯基的数量或浓度。DTNB 还是登革热病毒蛋白酶 protease (NS2B-NS3pro)和链霉菌蛋白酶的变构抑制剂。
DTNB reacts with the free sulfhydryl side chain of cysteine to form an S-S bond between the protein and a thionitrobenzoic acid (TNB) residue.
The main advantage of DTNB over alternative reagents (e.g., N-ethylmaleimide or iodoacetamide) is in the selectivity of this reagent and in the ability to follow the course of the reaction spectrophotometrically.
Modification of Enzyme with DTNB:
Modification of the SH groups of the enzyme is carried out by reacting 10 μL of 10 mM DTNB solution (about a 20-fold molar excess) at room temperature with 0.6 mL of enzyme solution (0.807 mg/mL) in 80 mM phosphate buffer, pH 8.0, containing 1 mM EDTA, which has been dialyzed previously against the same buffer solution for 24 h. The number of SH groups is estimated from the increase of absorbance at 410 nm using a molar extinction coefficient of 13,600 M
-1
cm
-1
for thionitrobenzoate ions liberated.
5,5'-二硫双(2-硝基苯甲酸) 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024-01-25 | HY-15915 | 5,5'-二硫双(2-硝基苯甲酸) | 69-78-3 | 1g | 200 |
| 2024-01-25 | HY-15915 | 5,5'-二硫双(2-硝基苯甲酸) | 69-78-3 | 10mM * 1mLin DMSO | 220 |
