Diethyl Phosphoramidate is an inhibitor of cholinesterase. It is toxic to houseflies and are used in insecticidal preparations. It also exhibits herbicidal and hydrolytic properties.
Diethyl phosphoramidate may be employed as reagent which mimics or imitates the actual chemical warfare agents. It may be used in the preparation of α-aminoalkyl phosphonates, via three-component Mannich type reaction.
Diethyl phosphoramidate is a chemical and biological toxin and its ultra-low level detection by Surface-Enhanced Raman Spectroscopy (SERS) is reported. It is reported as chemical warfare simulant.