N-(1-Anilinonaphthyl-4)-maleimide

red powder

N-(4-Anilino-1-naphthyl)maleimide is a fluorescent probe that can be used to detect thiol groups in proteins. N-(4-Anilino-1-naphthyl)maleimide reacts with thiol groups and immediately produces a stable fluorescent compound with the λex=355 nm, λem=448 nm[1].

[1] T Honda, et al. Characterization of the reactivity of sulphydryl groups in tryptophanase by a dual-monitoring high-performance liquid chromatographic system with a site-directed fluorescent reagent. J Chromatogr. 1986 Dec 26;371:353-60. DOI:10.1016/s0021-9673(01)94718-1
[2] S Chaen, et al. Fluorescence properties and contraction characteristics of ANM (N-(1-anilinonaphthyl-4)maleimide)-labeled rabbit psoas muscle fibers. J Biochem. 1985 Oct;98(4):939-47. DOI:10.1093/oxfordjournals.jbchem.a135374