3,3'-Dimethoxybenzidine dihydrochloride

o-Dianisidine (dihydrochloride) is a colorimetric peroxidase substrate that has been used as a substrate to semi-quantitatively measure lactate, uric acid , and glucose. It has also been used as a reagent for the detection of various metals, thiocyanates, and nitrites. This aromatic amine is initially colorless but turns to violet upon oxidization and is spectrophotometrically measured at a wavelength of 405 nm.

Off-white or grey tablets

o-Dianisidine (3,3′-dimethoxybenzidine) has been used as a component of GO (glucose oxidase) reagent, which is used for measuring the glucose content. It has also been used as a substrate to measure myeloperoxidase activity.

It is suitable for use in ELISA procedures. In neurohistochemistry o-Dianisidine dihydrochloride has demonstrated perikaryal labeling as well as retrograde and anterograde transport to the primary olfactory cortex. It has also been used for a paper based-micro fluidic device to semi-quantitatively measure lactate, uric acid, and glucose. 3,3'-Dimethoxybenzidine is used principally as an intermediate in the production of commercial bisazobiphenyl dyes for coloring textiles, paper, plastic, rubber, and leather.

Off-white powder.

Water soluble.

3,3'-Dimethoxybenzidine dihydrochloride is an ether substituted acidic organic salt. Reacts weakly as a acid to neutralize bases. May react with strong oxidizing agents or strong reducing agents.

Flash point data are not available for 3,3'-Dimethoxybenzidine dihydrochloride, but 3,3'-Dimethoxybenzidine dihydrochloride is probably combustible.

Confirmed carcinogen with experimental carcinogenic and tumorigenic data. Experimental reproductive data. Mutation data reported.When heated to decomposition it emits very toxic fumes of NO, and HCl.

[1] MAMORU SUGIURA  Kazuyuki H. A new colorimetric method for determination of serum glucose[J]. Clinica Chimica Acta, 1977, 75 3: Pages 387-391. DOI: 10.1016/0009-8981(77)90357-6
[2] M M MESULAM  D L R. Sensitivity in horseradish peroxidase neurohistochemistry: a comparative and quantitative study of nine methods.[J]. Journal of Histochemistry & Cytochemistry, 1979, 27 3: 763-773. DOI: 10.1177/27.3.113450
[3] 3,3’-Dimethoxybenzidine and dyes metabolized to 3,3’-dimethoxybenzidine: 3,3’-dimethoxybenzidine.[J]. Report on carcinogens: carcinogen profiles, 2011, 12: 164-165.