magenta b-galactosidase substrate
A substrate for β-Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm).
In histochemical staining, in blue-white selection of bacterial colonies with lacZ gene activity during cloning experiments.
A substrate for -Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm)
ChEBI: An indolyl carbohydrate that is the beta-D-galactoside of 3-hydroxy-1H-indole in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for
he presence of an enzyme, beta-galactosidase, which cleaved the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product.
X-gal is routinely used to detect transgene activity in situ.
