HIV-1 Tat Protein (47-57)

TAT (YGRKKRRQRRR) is derived from the transactivator of transcription (TAT) of human immunodeficiency virus-1 (HIV-1) and is a cell-penetrating peptide. TAT can increase the yields and the solubility of heterologous proteins

HIV-1 trans-activator of transcription (Tat) is an 11kDa protein, which regulates HIV-1 gene expression at the level of transcription and protein synthesis. It is located in the nucleus of infected cells. HIV-1 Tat functions as a transcriptional activator for the synthesis of full-length viral RNA. It induces RNA splicing. Tat is an RNA binding protein, which particularly recognizes trans activator response element (TAR) from the HIV-1 RNA molecule.

Synthetic peptide that includes the sequence responsible for the cellular uptake of the human immunodeficiency virus-1 Tat protein, consisting of the polycationic region 49–57. The peptide is part of the protein transduction domain (PTD) and was shown to enable the introduction of nucleic acids into cells.

HIV-1

[1] NICOLAS CHARNAY. Mechanism of HIV-1 Tat RNA translation and its activation by the Tat protein.[J]. ACS Applied Bio Materials, 2009: 74. DOI:10.1186/1742-4690-6-74.
[2] ASEN BAGASHEV Bassel E S. Roles and functions of HIV-1 Tat protein in the CNS: an overview.[J]. ACS Applied Energy Materials, 2013: 358. DOI:10.1186/1743-422X-10-358.
[3] NANCY MUELLER. The HIV-1 Tat Protein Enhances Splicing at the Major Splice Donor Site.[J]. ACS Applied Energy Materials, 2018. DOI:10.1128/JVI.01855-17.
[4] ESTEBAN CRUZ Veysel K. Synthesis and Enhanced Cellular Uptake In Vitro of Anti-HER2 Multifunctional Gold Nanoparticles.[J]. ACS Applied Energy Materials, 2019. DOI:10.3390/cancers11060870.
[5] ANDRé ZIEGLER Joachim S. Interaction of the protein transduction domain of HIV-1 TAT with heparan sulfate: binding mechanism and thermodynamic parameters.[J]. Biophysical journal, 2004, 86 1 Pt 1: 254-263. DOI:10.1016/S0006-3495(04)74101-6.