Description
Blasticidin S HCl (3513-03-9) is a peptidyl nucleoside antibiotic inhibiting protein synthesis in bacteria and eukaryotes.1Inhibits translation by trapping deformed tRNA on the ribosome.1The ABC-type transporter NppA1A2BCD is required for uptake of blasticidin S and other peptidyl nucleoside antibiotics inPseudomonas aeruginosaPA14.2Selection agent for bis, bsr and bsd transformed cells.3,4
Chemical Properties
White powder
Uses
Inhibits protein synthesis and induces apoptosis. Used to study protein synthesis at the level of peptide bond formation.
Uses
Blasticidin S hydrochloride is used to inhibit protein synthesis in prokaryotic and eukaryotic organisms. It also induces apoptosis.
Uses
Blasticidin S hydrochloride is a salt of blasticidin S, an amphoteric nucleoside produced by several species of Streptomyces, first reported in the late 1950s. Blasticidin S hydrochloride was originally isolated fby acetone precipitation. For historical reasons only the salt has been routinely available to researchers. Both the salt and free base are freely water soluble. Blasticidin S is an antifungal agent with particularly potent activity against the rice pathogen, Piricularia oryzae, for which it was used commercially for some time in Japan. Blasticidin S inhibits protein synthesis and is active against bacteria, tumour cell lines and nematodes. More recently, blasticidin S has been used as a marker for strain manipulations.
General Description
Nucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for selection of cells carrying plasmids conferring blasticidin resistance. Blasticidin resistance is conferred by the blasticidin S deaminase gene (
bsr) that converts blastisidin S to a nontoxic deaminohydroxy derivative.
References
[1] EGOR SVIDRITSKIY. Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110 30: 12283-12288. DOI:
10.1073/pnas.1304922110[2] DANIEL PLETZER. The Pseudomonas aeruginosa PA14 ABC Transporter NppA1A2BCD Is Required for Uptake of Peptidyl Nucleoside Antibiotics.[J]. Journal of Bacteriology, 2015, 197 13: 2217-2228. DOI:
10.1128/jb.00234-15[3] MASAHITO ASADA. Transfection of Babesia bovis by Double Selection with WR99210 and Blasticidin-S and Its Application for Functional Analysis of Thioredoxin Peroxidase-1.[J]. PLoS ONE, 2015: e0125993. DOI:
10.1371/journal.pone.0125993[4] ROHINI GARG Ayub Q. Hemoglobin transforms anti-inflammatory Salmonella typhi virulence polysaccharide into a TLR-2 agonist.[J]. Journal of immunology, 2010, 184 11: 5980-5987. DOI:
10.4049/jimmunol.0903512
