Description
DSPG is used in FDA approved drugs. It is an anionic phospholipid derivative. DSPG has been used to form an ionic complex with amphotericin B and encapsulate it into small unilamellar liposomes with significantly reduced toxicity (AmBisome?). DSPG-Na is a lipid reagent, which is useful in drug formulation, DNA & RNA delivery.
in vitro
Three liposome formulations were investigated: HSPC:Chol (L-EPI; 5:4 molar ratio), HSPC:Chol:DSPG (D-EPI; 5:4:1 molar ratio), and HSPC:Chol:DSPG:DSPE-mPEG(2000) (S-EPI; 5:4:1:0.3 molar ratio). Small unilamellar liposomes were prepared by the modified thin-film hydration method with extrusion through polycarbonate filters, and EPI was remote loaded into liposomes by the transmembrane ammonium sulfate gradient method._x000D_
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Reference: J Liposome Res. 2012 Mar;22(1):80-8. https://pubmed.ncbi.nlm.nih.gov/22022836/
in vivo
Vaccination of atherosclerotic mice on a western-type diet with DSPG-liposomes encapsulating an LDL-derived peptide antigen significantly reduced plaque formation by 50% and stabilized the plaques, and reduced serum cholesterol concentrations. These results indicate that DSPG-liposomes have potential as a delivery system in vaccination against atherosclerosis._x000D_
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Reference: J Control Release. 2018 Dec 10;291:135-146. https://pubmed.ncbi.nlm.nih.gov/30365993/
